硫氧还蛋白还原酶活性检测试剂盒在恶性肿瘤辅助诊断中的价值

Clinical value of Thioredoxin Reductase Detection Kit for diagnosis of cancers

目的探讨硫氧还蛋白还原酶(TR)活性检测试剂盒在恶性肿瘤辅助诊断中的价值。方法检测不同地区、不同检验机构1122例健康人群TR活性,差异有无统计学意义;检测84例恶性肿瘤患者TR活性,并与健康人群比较,差异有无统计学意义,通过ROC曲线对检测结果进行分析评价。结果北京体检机构的TR活性均值为(2.6±4.1)U/mL,武汉体检机构的TR均值为(2.6±3.7)U/mL,湖北中医药大学附属医院的TR均值为(2.4±3.5)U/mL,各组间TR活性水平差异无统计学意义(P>0.05);肿瘤组TR活性均值为(9.2±6.4)U/mL,健康组TR活性为(2.5±3.7)U/mL,两者间差异有统计学意义(P<0.05)。固定健康组和肿瘤组的样本比例为4:1,不同的样本量间TR活性差异无统计学意义(P>0.05)。ROC曲线下面积为0.812±0.029。结论组间均数比较U检验和ROC曲线评价均显示,TR活性检测试剂盒用于与异常增生密切相关的肿瘤生长的临床血样检测具有重要的实用价值。

Objective To evaluate the clinical value of Thioredoxin Reductase（TR） Detection Kit for diagnosis of cancers.Methods Serum levels of TR were detected by TR Detection Kit in 1 122 healthy subjects from different regions and medical institutions and 84 patients with different cancers.Differences of the detected results between each group were compared and ROC curve was prepared to evaluate the specificity and sensitivity of this detection.Results Serum levels of TR in the three healthy subgroups were（2.6±4.1）,（2.6±3.7） and（2.4±3.5）U/mL,with no statistical difference between the three subgroups（P〉0.05）,and the difference was barely significant between 3 samples with fixed ratio（P〈0.05）.Serum levels of TR in cancer group was（9.2±6.4）U/mL,with statistical difference with the（2.5±3.7）U/mL in healthy group（P〈0.05）.There was no statistical difference between The area under ROC curve was 0.812±0.029.Conclusion Detection of TR by TR Detection Kit could be valuable for clinical diagnosis of cancers.