Treg、Ki67和VEGF在结直肠癌中的表达及意义

Expression and clinical significance of Treg,Ki67 and VEGF in colorectal cancer

目的探讨调节性T细胞(Treg)、增殖细胞核抗原(Ki67)和血管内皮生长因子(VEGF)在结直肠癌(CRC)患者肿瘤微环境中的表达,以及Treg与Ki67、VEGF的相关性。方法采用流式细胞术测定21例CRC患者的外周血以及肿瘤微环境和24例健康人外周血中Treg的表达,同时应用免疫组化法检测CRC组织中Ki67和VEGF的表达。结果 CRC患者肿瘤微环境中Treg比较[(26.84±10.22)%]明显高于外周血单个核细胞(PBMC)[(7.01±3.08)%],差异有统计学意义(P<0.01)。Treg细胞表达频率在DukesC期和D期(晚期)的肿瘤组织中[(35.01±6.78)%],明显高于DukesA期和B期(早期)[(24.40±8.95)%],差异有统计学意义(P<0.05)。相关分析表明Treg与Dukes临床分期呈正相关(r=0.511,P<0.05);Treg与Ki67的表达呈正相关(r=0.455,P<0.05);Treg与VEGF的表达呈正相关(r=0.198,P>0.05),但无统计学意义。结论 Treg与Ki67、VEGF的相互作用可能促进了肿瘤的发生、发展与转移,对CRC的预后评价有着重要的参考价值。

Objective To investigate the relationship of the expression and clinical significance between regulatory T cells（Treg）,proliferating cell nuclear antigen（Ki67） and vascular endothelial growth factor（VEGF） in colorectal cancer（CRC）.Methods The proportion of Treg was evaluated by flow cytometry in the peripheral blood and tumor microenvironment in 21patients with CRC and 24healthy donors.The expression of Ki67and VEGF in CRC tissues were detected by immunohistochemistry assay.Results The percentage of Treg in tumor microenvironment was significantly higher than in peripheral blood mononuclear cell（PBMC）[（26.84±10.22） %vs（7.01±3.08） %,P〈0.01 ].Especially in tumor tissues,the frequency of Treg was markedly increased in advanced clinical stages（Dukes’C and D stage） compared to the early clinical stages（Dukes’A and B stage）[（35.01±6.78） %vs（24.40±8.95） %,P〈0.05 ].The frequency of Treg was positively correlated with clinical stages（r=0.511,P〈0.05） and Ki67 expression（r=0.455,P〈0.05）,but was not significantly related to the expression of VEGF in tumor（r=0.198,P〉0.05）.Conclusion The correlation of Treg,Ki67and VEGF might be involved in the oncogenesis,development and metastasis of CRC,and could be used as prognostic indicators of CRC.