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《荧光原位杂交分析染色体易位估算辐射生物剂量技术方法》解读 被引量:2

Explanation of Method of Dose Estimation Using Chromosome Translocation with Fluorescence in Situ Hybridization
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摘要 国家职业卫生标准——《荧光原位杂交分析染色体易位估算辐射生物剂量技术方法》是在收集整理、全面阅读了与该标准有关的国内外文献资料和我国现行有效的放射性疾病诊断标准,将资料汇总分析后经实验室反复验证的基础上制定的。此标准主要用于早先事故受照人员的生物剂量估算,为临床放射损伤的诊断提供科学依据。为了使放射性疾病临床工作者更好地理解、贯彻执行该标准,该文对标准的相关内容进行了解读。 National occupational health standard--Method of Dose Estimation Using Chromosome Translocation with Fluorescence in Situ Hybridization has been developed, which based on comprehensive collection, reading and analysis of relevant literature both abroad and domestic and the existing diagnostic criteria for radiation diseases of the China, and the repeated experimental verification. This standard is mainly applied for dose estimation for individuals who previously exposed to irradiation by accident, and provide the scientific diagnosis of radiation sickness. To better understand and implement it, the contents of this standard were interpreted in this article.
出处 《国际放射医学核医学杂志》 2012年第4期245-248,共4页 International Journal of Radiation Medicine and Nuclear Medicine
基金 基金项目:卫生部标准研究课题(2009-09-01)
关键词 原位杂交 荧光 易位 遗传 辐射剂量 职业卫生标准 In situ hybridization, fluorescence Translocation, genetic Radiation dosage Occupational health criteria
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参考文献19

  • 1中华人民共和国卫生部.GB/T28236-2011染色体畸变估算生物剂量方法.北京:中国标准出版社,2012.
  • 2中华人民共和国卫生部.WS/T204-2001用稳定性染色体畸变估算职业受照者剂量的方法.北京:中国标准出版社,2002.
  • 3Pinkel D, Straume T, Gray JW. Cytogenetic analysis using quanti- tative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci U S A, 1986, 83(9): 2934-2938.
  • 4FanYS.分子细胞遗传学-技术与应用.刘青杰,译.北京:科学出版社,2007:3-7.
  • 5International Atomic Energy Agency. Cytogenetic analysis for radi- ation dose assessment. A manual technical report series No. 405. Vienna: IAEA, 2001: 109-115.
  • 6Lucas JN, Awa A, Straume T, et al. Rapid translocation frequency analysis in humans decades after exposure to ionizing radiation. Int J Radiat Biol, 1992, 62( 1 ): 53-63.
  • 7Bauchinger M, Braselmann H, Savage JR, et al. Collaborative exer- cise on the use of FISH chromosome painting for retrospective bio- dosimetry of Mayak nuclear-industrial personnel. Int J Radiat Biol, 2001, 77(3): 259-267.
  • 8Camparoto ML Ramalho AT, Natarajan AT, et al. Translocation analysis by the FISH-painting method for retrospective dose recon- struction in individuals exposed to ionizing radiation 10 years after exposure. Mutat Res, 2003, 530( 1-2): 1-7.
  • 9刘青杰,陈德清,陈晓宁,Julie R.Korenberg,姜恩海.多色荧光原位杂交技术的建立及其在早先受照射者剂量重建中的应用[J].中华放射医学与防护杂志,2003,23(2):77-82. 被引量:14
  • 10陆雪,吕玉民,封江彬,陈德清,刘青杰.用染色体涂染方法对早先辐射受照人员进行回顾性照射剂量估算[J].癌变.畸变.突变,2008,20(2):135-138. 被引量:6

二级参考文献26

  • 1Cigarran S, Barrios L, Barquinero JF, et al. Relationship between the DNA content of human chromosomes and their involvement in radiation-induced structural aberrations analysed by painting. Int J Radiat Biol,1998,74:449-455.
  • 2Johnson KL, Nath J, Pluth JM,et al. The distribution of chromosome damage, non-reciprocal translocations and colonial aberrations in lymphocytes from Chernobyl clear-up workers. Mutat Res, 1999,439:77-85.
  • 3Chen D ,Zlmng C. A simple and convenient method for gaining pure populations of lymphocytes at the first mitotic division in vitro. Mutat Res,1992,282:227-229.
  • 4Korenberg JR, Chen XN, Sun ZG, et al. Human genome anatomy: BACs integration the genetic and cytogenetic maps for bridging genome and biomedicine. Genome Res, 1999,9: 994-1001.
  • 5Korenberg JR, Chen XN. Human cDNA mapping using a high-resolution R-banding technique and fluorescence in situ hybridization. Cytogenet Cell Genet, 1995,69:196-200.
  • 6Pinkel D, Straume T, Gray JW. Cytogenetic analysis quantitative high sensitivity, fluorescence hybridization. Pro Natl Acad Sci USA, 1986,83: 2934-2938.
  • 7Salassidis K, Georgiadou-Schumacher, Braselmann H, et al. Chromosome painting in highly irradiated Chernobyl victims: a follow-up study to evaluate the stability of symmetrical translocations and the influence of clonal aberrations for retrospective dose estimation. Int J Radiat Biol, 1995,68 :257-262.
  • 8Natarajan AT, Vyas RC, Wiegant J, et al. A cytogenetic follow-up study of the victims of a radiation accident in Goiania(Brazil). Mutat Res,1991,247:103-111.
  • 9Xiao Y, Darroudi F, Gfigorova M, et al. Induction and persistence of chromosome exchanges in mouse bone marrow cells following whole-body exposure to X-rays. Int J Radiat Biol, 1999,75 : 1119-1128.
  • 10Tucker JD, Breneman JW, Briner JF, et al. Persistence of radiation-induced translocation in rat peripheral blood determined by chromosome painting. Environ Mol Mutagen, 1997,30: 264-272.

共引文献38

同被引文献24

  • 1卢圣栋.现代分子生物学实验技术[M].北京:高等教育出版社,1992:147.
  • 2董玉玮,邱龙,李培青,刘焕民,庞永红,朱必才.PCR和随机引物标记探针的方法比较[J].生物学杂志,2007,24(1):63-66. 被引量:7
  • 3Weier H-UG,Lucas JN,Poggensee M,et al. Two-colorhybridization with high complexity chromosome-specific probesand a degenerate alpha satellite probe DNA allows unambiguousdiscrimination between symmetrical and asymmetricaltranslocations[J]. Chromosoma,1991,100 (6):371-376.
  • 4Chen DQ,Zhang CY. A simple and convenient method forgaining pure populations of lymphocytes at the first mitoticdivision in vitro[J]. Mutat Res,1992,282(3):227-229.
  • 5Mosesso P,Palitti F,Pepe G,et al. Relationship betweenchromatin structure,DNA damage and repair following X-irradiation of human lymphocytes [J]. Mutat Res,2010,701(1):86-91.
  • 6Johnson KL,Nath J,Pluth JM,et al. The distribution ofchromosome damage,non-reciprocal translocations andcolonial aberrations in lymphocytes from Chemobyl clear-upworkers [J]. Mutat Res,1999,439 (1):77-85.
  • 7Willard HF. Chromosome-specific organization of human alphasatellite DNA [J]. Am J Hum Genet,1985,37 (3):524-532.
  • 8Hayden KE, Strome ED,Merrett SL,et al. Sequence associatedwith centromere cometency in human genome[J]. Mol Cell Biol,2013,33 (4):763-772.
  • 9Lamadrid AI,Garcia O,Delbos M,et al. PCC-ring inductionin human lymphocytes exposed to gamma and neutron irradiation[J]. J Radiat Res,2007,48 (1):1-6.
  • 10Viral A,Fenech M,Thierens H. The micronucleus assay as abiological dosimeter of in viro ionizing radiation esposure[J].Mutagenesis,2011,26 (1):11-17.

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