牛血清白蛋白诱导免疫性心肌炎小鼠模型的建立

Establishment of Autoimmune Myocarditis Model Induced by Immunization of Mice with Bovine Serum Albumin

目的探讨异种动物蛋白致小鼠免疫性心肌炎的可能性，为研究自身免疫性心肌炎提供新的动物模型。方法实验组和对照组小鼠分别于0、3、5、10d经腹腔皮下注射0．5ml的10％牛血清白蛋白和生理盐水。分别于第14日和第28日进行检测。结果所有实验组小鼠在初次免疫后第14日出现不同程度的心肌炎病理改变，光镜下主要表现为心肌间质淋巴细胞浸润，局灶性心肌坏死和局灶性出血，并同时存在心内膜炎和心外膜炎。电镜下见心肌细胞及线粒体肿胀、肌丝溶解和淋巴细胞浸润。免疫鼠脾脏淋巴细胞升高，心肌酶值升高。在初次免疫后第28日上述炎症及心肌坏死减轻，脾脏淋巴细胞较前下降，血清心肌酶值较前无明显变化。结论用牛血清白蛋白能够诱导小鼠产生自身免疫性心肌炎，该动物模型病理改变典型。

Objective To approach feasible of autoimmune myocarditis induced by immunization of mice with bovine serum albumin, to proved a new animal model of autoimmune myocarditis. Methods Experimental group and control group were subcutaneously treated with 0.5 ml 10% bovine serum albumin and physiological saline respectively on day 0, 3, 5, and 10, and then related tests were conducted on day 14 and 28 to confirm if the model was established successfully. Results On day 14 after the first immunization with bovine serum albumin, all BALB/c mice from the experimental group were developed with myocardial interstitial lymphocytic infiltrates, focal myocardial necrosis and haemorrhages, accompanying with endocarditis and epicarditis. Under the electron microscope： It was found myocardial cell and mitochondria swelling, muscle fibers dissolved and lymphocyte infiltration. A high titer of myocardium creatase and more lymphocyte were detected in the immunized mice. On day 28 after the first immunization with bovine serum albumin, the inflammation and myocardial necrosis were not seriously, a same titer of myocardium creatase and less lymphocyte were detected in the immunized mice. Conclusion Autoinmune myocarditis can be induced by immunization of mice with bovine serum albumin, pathological changes of the animal model arre typical, the rate of colony is high.