摘要
目的探讨锌对甲基汞急性染毒大鼠组织及血液各氧化指标的变化。方法大鼠随机分为锌+甲基汞组、甲基汞组和对照组,锌+甲基汞组大鼠每目腹腔注射不同剂量硫酸锌,剂量分别为2.86、5.72、8.58、11.44mg/kg,第6日甲基汞急性染毒,第7日处死,取大鼠肝、脑、肾及血液分别测定谷胱甘肽过氧化物酶(GSH-Px)活性、超氧化物歧化酶(SOD)活性。结果随着锌剂量的增加,大鼠组织和血液中GSH—Px活性降低,GSH—Px活性与锌剂量呈显著负相关;随着锌剂量的增加,大鼠组织和血液中SOD活性增强,锌+MeHg实验组中SOD活性与锌剂量呈显著正相关关系。结论锌能有效预防甲基汞染毒大鼠的机体损伤。
Objective To investigate the changes of tissue and hematological oxidation in acute methyl mercury-contaminated rat. Method The total of rats were randomly divided into three groups, zinc+methyl mercury groups, methyl mercury exposure group and control group, zinc+methyl mercury groups were treated with different doses of ZnSO4 (2.86, 5.72, 8.58, 11.44 mg/kg) by intraperitoneal injection every day. On the 6th day, the rats in all groups except control group were exposed to methylmecury by oral administration. All the rats were sacrificed on the 7 th day, the activity of super- oxide dismutase (SOD) and glutathione peroxidase (GHS-Px) in the liver, brain, kidney and blood were determined. Results With the zinc+methyl mercury groups, compare to the treatment of methylmecury, the activities of SOD significantly increased (P〈0.05) and GSH-Px significantly decreased (P〈0.05) in the tissues and blood, and shown a significant positive correlation. Conclusion The Zinc has signifi- cant inhibitive effect on methylmercury-induced oxidative damage in rats.
出处
《实验动物与比较医学》
CAS
2012年第4期304-307,共4页
Laboratory Animal and Comparative Medicine
关键词
锌
甲基汞
超氧化物歧化酶
谷胱甘肽过氧化物酶
金属硫蛋白
Zinc
Methylmercury
Glutathione peroxidase
Lipid peroxide
Superoxide dismutase
Metallthionein