顺铂致小鼠急性肾功能损伤模型的建立

Establishment of Acute Renal Impairment Model Induced by Cisplatin in Mice

目的建立顺铂致小鼠急性肾功能损伤动物模型。方法腹腔注射顺铂10mg／kg，以造成小鼠急性肾损伤模型，空白对照组给予等量生理盐水。给药后第8日，戊巴比妥钠麻醉小鼠，取血，检测血清中肌酐（CRE）、尿素氮（BUN）水平，并对各组动物的肾脏组织进行病理学检查。结果模型组动物血清CRE、BNU显著升高，肾系数降低，肝脏系数增加，体重增长及免疫器官重量均受到抑制。肾脏病理结果显示，模型组动物肾小管上皮细胞浑浊肿胀，结构不清，有轻度纤维增生，肾间质有炎性细胞浸润灶。结论7—8周龄成年小鼠一次性腹腔注射给予川页铂10mg／kg可成功建立急性肾损伤动物模型．

Objective To establish a model of acute renal function impairment induced by cisplatin in mice. Method The mice were injected with 10 mg/kg cisplatin in the 1st day to establish acute renal impairment model. The mice in the control group were dosing commensurable 0.9% NaCl saline solution. The mice were taken eyeballs for blood collection after anesthesia by pentobarbitalin the 8th day, and the serum CRE, BUN levels were measured. Pathological analysis was taken to compare the changes of the renal tissues between model group and control group. Results CRE and BUN levels were raised obviously in the model group, renal index, body weight and immune organic weight were reduced obviously in the model group, at the same time, the liver index was raised in the model group. The result of the pathological analysis was showed that the degeneration and necrosis of renal tubular epithelial cells were observed in the model group, and there are some inflammatory infiltrates foci in renal mesenchymal cells in the model group. Conclusion Acute renal impairment model could be established by injected 10 mg/kg cisplatin in 32-40 g mice.