乳腺癌中AKT激活与耐药蛋白表达相关性

Correlation of AKT activation with drug resistance protein expression in breast cancer

目的明确乳腺癌中Akt的过度表达与P-gp、GST-π、TopoⅡ三种耐药蛋白的相关性。方法采用免疫组织化学方法检测260例乳腺癌中Akt和P-gp、TopoⅡ、GST-π的表达情况。结果乳腺癌中Akt、GST-π和TopoⅡ的表达与肿瘤大小相关,随着肿瘤体积增大,AKT、GST-π和TopoⅡ的阳性表达率增高;Akt和P-gp在伴有淋巴结转移的病例中高表达,且随着淋巴结的数目增多Akt表达越来越高;Akt、P-gp和GST-π的阳性表达患者预后差;Akt与P-gp、GST-π、Topo-Ⅱ阳性表达之间均呈显著相关性。结论信号转导途径中Akt的激活与P-gp、GST-π、TopoⅡ三种耐药蛋白有相关性,AKT有可能在乳腺癌多药耐药的发生机制中起到一定的作用。

Objective To explain whether over-expression of the Akt gene is correlated to the expressions of P-gp, GST-π and TopoⅡ in breast cancer. Methods SP immunohistochemical method was used to examine the expressions of Akt, P gp, GST-π and Topo Ⅱ in 260 samples of primary breast cancer. Results Akt, GST- and Topo π expressions in breast cancer were positively associated with tumor size. High expression of Akt and P-gp were seen in the cases with lymph node metastasis, and the expression of Akt became higher and higher with the increase in the number of lymph nodes. The positive expressions of Akt, P-gp and GST-= indicated poor prognosis. There were significant correlations between Akt and positive expressions of P-gp, GST-πand Topo Ⅱ. Conclusion Activation of Akt in the signal transduction pathway is correlated with resistance proteins P-gp, GST-π, and Topo Ⅱ. AKT may play a certain role in the mechanism of multidrug resistance in breast cancer.