水飞蓟素对小鼠肝损伤保护作用及机理研究

The study of Prorection effect of model silmyarin on hepatic injured in mice

目的:探讨水飞蓟素对小鼠免疫性肝炎及实验性肝损伤保护作用。方法:小鼠iv卡介苗(BCG)5×106个菌/鼠;11天后再iv脂多糖(LPS)7.5pg/只,造成免疫性肝炎模型及对小鼠四氯化碳(CCL4)和硫代硫酰胺(TAA)所引起实验性中毒模型;观察水飞蓟素对免疫性肝炎及四氯化碳和硫代硫酰胺致实验性肝损伤小鼠的保护作用。结果:水飞蓟素和联苯双酯两药能明显降低免疫性肝炎及实验性肝损伤模型小鼠血清谷丙氨酸转氨酶(ALT),谷草转氨酶(AST)的作用(P<0.01);血清的超氨化物岐化酶(SOD)的活性升高、丙二醛及机理(MDA)和一氧化氮(NO)含量明显降低。结论:水飞蓟素对小鼠免疫性肝炎及实验性肝损伤有保护作用。

Abstract： Objective： To observe the study the effect prorection of model silmyarin on Immunological hepatitis and experimental hepatotoxicity in mice. nothods ： The mouse model of immunological hepatits was established by intravenously injecting of BCG （5 × 106bacilli/mouse） and after 11 days intravenously injecting of lipopdysaccharide （7. 5μg/mouse ） and experimental hepatotoxicity mice model induced by Carbon Tetrchloride （ CCL4 ） and Thioacetamide （TAA）. The research of effect Prorection of machemism silmyarin on Immuological Hepatitis and experimental hepatotoxicity in mice. Results： The effected of silmyarin significantly decrease the liver of serum ALT, AST in mouse immulogical hepatitis model. The silmyarin could obviously increase the activity by SOD in ser- um of mice and the content of MDA could obviously decrease of serum. Conlusious： silmyarin could frotect mice from immunological liver injury and experimental hepatotoxicity.