细胞命运转变——谱系重编程技术研究进展

Cell fate switch:Lineage reprogramming

体细胞核移植和诱导多能干细胞技术表明已分化的体细胞可以转变命运。最近的研究再一次验证了成熟体细胞可以通过外源转录因子的导入,直接重编程为其他类型的体细胞或祖细胞。这种重编程技术称为谱系重编程(Lineage reprogramming)。这项技术不仅在再生医学领域具有广阔的应用前景,而且在动物生物技术中也应用广泛。它不但避免了伦理争议,还提供了便利的重编程方法,同时也为基因表达调控的研究提供了重要的手段。文章从谱系重编程的方式、谱系重编程的特点及应用前景等3个方面进行了综述,旨在对相关领域的研究人员起到借鉴作用。

It has been demonstrated that mature cells could switch their fate by the technologies for reprogramming,such as somatic cell nuclear transfer and induced pluripotent stem cells.Recently,this conclusion was further confirmed.It was found that mature differentiated cells could be directly converted into other somatic cells or progenitors with some defined transcription factors.This technology is called lineage reprogramming,which provides an attractive novel alternative to regenerative medicine and animal biotechnology.It is a more convenient and more effective system with less ethical issues.Moreover,lineage reprogramming technology could also facilitate researches on regulation of gene expression.This review highlights the procedures of reprogramming,its characteristics,and significant promise in biomedical applications