QuEChERS-HPHPLC联用法检测蔬菜中有机磷农药多残留分析

DETECTION OF ORGANOPHOSPHORUS PESTICIDE RESIDUES IN VEGETABLE USING QUECHERS-HPLC

建立了QuEchERS-HPLC联用法检测蔬菜中有机磷农药多残留的方法。改进了QuEchERS前处理方法,提高回收率及结果准确性,然后运用HPLC进行分析检测,根据有机磷农药的差异,得出其最小检出限在0.02μg/kg～55.75μg/kg之间,定量限0.1μg/kg～189.23μg/kg,18种有机磷农药要在定量限浓度和四倍定量限浓度2个加标水平的回收率均在70%～120%范围,相对标准偏差低于20%,试验表明本方法适用于蔬菜中有机磷农药的多残留检测分析。

The method of detected Organophosphorus Pesticide Residues in Vegetable Using QuEChERS- HPLC was established. Through improvement of the QuEchERS pretreatment methods, recovery and accuracy was improved well. Then by using HPLC analysis detection, according to the difference of organo- phosphorus pesticide, the minimum detection limit was obtained as between 0.02-55.75μg/kg, LOQ as between 0.1-189.23μg/kg. At two labeled level of LOQ concentration and four times the concentration of LOQ, recovery of 18 organophosphorus pesticide was from 70% to 120%, and the relative standard de- viation was less than 20 %. It shows that this method is applicable to detect organophosphorus pesticide residue of vegetables.