不同库外保护时间和冷藏时间对滞育卵冷藏浸酸种孵化率的影响

Effect of protection and refrigeration time outside storeroom on hatching rate of acid-treated diapause silkworm (Bombyx mori) eggs

【目的】验证库外保护时间和冷藏时间对滞育卵冷藏浸酸种孵化率的影响程度,为今后做好蚕种保护冷藏浸酸工作提供理论依据。【方法】以"两广二号"正、反交一代杂交种为研究对象,将不同库外保护时间(5～60d,25℃)和不同内库冷藏时间(100～175d,3～5℃)的滞育卵,在同一时间以标准浸酸处理后统计其实用孵化率和孵化整齐度。【结果】蚕种冷藏时间100～175d、库外保护时间10～60d时,蚕种实用孵化率为90.33%～97.33%,符合蚕种质量标准规定的实用孵化率在90.00%以上的要求。冷藏时间100～175d、库外保护时间5～60d的蚕种孵化并不在同一日内完成,其中,库外保护5～10d的蚕种孵化比库外保护时间更长(15～60d)的蚕种孵化时间相对早且集中,即库外保护时间越长,孵化时间相差程度越明显。【结论】库外保护时间长短对滞育卵冷藏浸酸种实用孵化率影响不明显,但对蚕种的孵化整齐度有影响,尤其是库外保护时间在10d以内的蚕种表现更为明显。

[Objective]The present experiment was carried out to investigate the effects of different protection and refrigeration time outside storeroom on hatching rate of acid-treated diapause silkworm eggs in order to provide references for better protecting and storing acid-treated silkworm eggs in future. [Method]The hatching rate and uniformity of acid-trea- ted diapause eggs, obtained from the cross and reciprocal cross F1 hybrids of silkworm variety Liangguang 2,were investigated after protecting them outside the storeroom at 25℃ for 5-60 days and refrigerating at 3-5℃ for 100-175 days. [Result]After protected for 10-60 days and refrigerated for 100-175 days, the practical hatching rate of silkworm eggs was 90.33-97.33%, which met the demand of silkworm eggs quality standard （above 90.00%）. Hatching of silkworm eggs was not finished on the same day after refrigerated for 100-175 days and protected for 5-60 days. Silkworm eggs protected for 5-10 d hatched earlier and more uniform than that protected for longer time （15-60 days）, that is, the longer the eggs protected, the more difference in their hatching time. [Conclusion]Therefore, protection time outside storeroom did not affect practical hatching rate but affected the uniformity of hatching rate, which was more obvious for the eggs protected within10 days.