一株丁草胺降解菌的分离鉴定(英文)

Isolation and identification of a degrading bacterium to herbicide butachlor

通过富集培养技术结合高效液相色谱法检测,从长期受丁草胺污染的污泥中筛选出1株丁草胺降解菌,命名为Y-1。经形态特征、生理生化特征和16S rDNA序列同源性分析,将该菌株鉴定为假单胞菌属(Pseudomonas sp.)。通过研究Y-1在不同条件下的降解特性发现,降解丁草胺的最优条件为:丁草胺初始浓度20 mg/L、接种量5%、pH值7、培养温度30℃。在最优环境条件下培养7 d,Y-1能降解培养液中76%的丁草胺,显示了良好的降解能力。

From the sludge polluted by butachlor for a long time,the degrading bacterium to butachlor was selected through enrichment culture with combination of High Performance Liquid Chromatography（HPLC） detection and then named as Y-1.Through the analysis of morphological characteristics,physiological and biochemical characteristics,and 16S rDNA sequence homology,Y-1 was identified as Pseudomonas sp..Through the research of the degradation character of Y-1 in different ambient conditions,the optimal conditions were found as follows： the initial concentration of butachlor of 20 mg/L,inoculum concentration of 5%,pH of 7 and temperature of 30℃.The degradation rate of Y-1 to butachlor reached 76% after the cultivation for 7 days in optimal conditions,which showed the excellent degradation ability.