阿伐他汀诱导人胆管癌QBC939细胞凋亡的研究

Effects of Atorvastatin on the proliferation and apoptosis in human cholangiocarcinoma QBC939 Cells

目的观察阿伐他汀(ATV)对人胆管癌QBC939细胞增殖及凋亡的影响。方法应用细胞培养技术培养QBC939细胞,经不同浓度的阿伐他汀处理后,以MTT法检测QBC939细胞的存活率,通过光镜、倒置显微镜及琼脂糖凝胶电泳检测胆管癌细胞凋亡的形态学特征、生化学特征。结果在阿伐他汀的作用下,QBC939细胞的生长、增殖变的相对缓慢,凋亡的细胞出现膜小泡、凋亡小体等特征性改变;DNA电泳呈现典型的梯状条带。结论阿伐他汀可抑制胆管癌细胞的生长、增殖,并可诱导细胞发生凋亡,且其作用呈剂量效应关系。

Objective To investigate the effects of Atorvastatin on the proliferation and apoptosis in human cholangiocarci- noma QBC939 cells. Methods The human cholangiocarcinoma QBC939 cell line was incubated with different concentrations of a- torvastatin for 48h. MTT assay for cell growth and viability, Cell apoptosis was ascertained by cell morphology under light-,and inverted microscope,and DNA electrophoresis. Results Atorvastatin can inhibits growth and proliferation of QBC939 cells,and al- so induces cell apoptosis. Conclusion Atorvastatin can inhibits proliferation and induce apoptosis of human eholangioearcinoma QBC939 cells.