不同处理方法对血小板表面活化标志CD62p流式检测结果的比较

Comparison of Flow Cytometry Detection Results of Platelet Activation Marker CD62p by Different Treatment Methods

目的探讨不同处理方法对血小板表面活化标志CD62p流式检测结果的影响。方法采集作者血液中心单采血小板浓缩液5 ml,用贫血小板血浆调整血小板计数为(200～250)×109/L(待测PRP,n=6),分别采用不洗涤不固定不加活化抑制剂与洗涤、固定、加活化抑制剂进行标本处理,流式细胞仪检测血小板活化标志CD62p的变化。结果 CD62p的表达与标本的处理方法有关,但4组之间没有统计学差异(P>0.05)。其中不洗涤不固定不加活化抑制剂组CD62p测定值为(40.47±2.54)%,低于其它3组检测值[(46.35±2.60)%、(47.54±3.47)%、(45.85±2.88)%]。结论流式细胞术检测血小板活化标志物CD62p中,标本处理步骤越少其活化率就越低,以不洗涤不固定不加活化抑制剂为最好。

Objective To study the effect of different treatment on the detection of platelet activation marker CD62p by flow cytometry.Methods 5 ml of platelets concentrates were obtained from healthy donors by Amicus sparator.The platelet counts were adjusted by platelet-poor plasma to（200-250）×10^9/L（n=6）.Then the specimens were treated with four different methods（no wash and no fix,wash but no fix,no wash but fix,no wash and no fix but being added with PGE1） respectively.The treated specimens were detected by flow cytometry to analyze the changes of CD62p expression.Results CD62p expression was related with the reatment process of the specimens.There was no significant difference among the four groups（P〉0.05）.The CD62p expression of no wash and no fix group（40.47±2.54）% was lower than the other three groups（（46.35±2.60）%,（47.54±3.47）%,（45.85±2.88）%）.Conclusion In the detection of CD62p by flow cytometry,the less the specimen process steps,the lower the activation rate.The best treatment method is no wahs,no fix and no additional PGE1.