178株酵母样真菌的鉴定及药敏试验分析

目的:了解住院患者酵母样真菌的感染类型以及对常用抗真菌药物的耐药情况,为临床诊疗提供合理的用药依据。方法:采用常规真菌培养方法,将标本接种于沙保罗培养基,待细菌长出后接种科玛嘉显色平板联合半自动鉴定及药敏分析仪进行鉴定及药敏分析。结果:178株酵母样真茵中,白色念珠茵140株(78.7%),其次为热带念珠茵19株(10.7%)和光滑念珠茵16株(9.0%),其中,呼吸道标本痰液和咽拭子酵母样真茵检出率最高,达87%,其次是尿液7.3%。其中对两性霉素B、制霉菌素敏感性最高100%,对氟胞嘧啶敏感性达95%以上,对氟康唑和伊曲康唑的敏感性最低。结论:临床标本中酵母样真茵以白色念珠茵为主,呼吸道是酵母样真茵感染最常见的部位,大部分患者都是免疫力低下的老年人,长期使用抗生素或免疫抑制剂是感染真菌及药物敏感性降低的主要原因。

Objective:to our hospital patients yeast sample fungal infection type and to commonly used the antifungal drugs and drug resistance of clinical diagnosis and treatment to provide rational drug use basis.Methods:using conventional fungi cultivation method,the specimen vaccination albright Paul culture medium,stay long after the bacteria have instead been growing families and fine show color plate joint semi-automatic stars identified and the identification of drug sensitive analyzer and medicine sensitivity analysis.Results:178 strains of yeast kind of really bitter,white beads feed 140 strains(78.7%),followed by a tropical beads feed 19 strains(10.7%)and smooth beads feed 16 strains(9.0%),among them,the respiratory tract sputum specimens and throat swabs sample is highest rate from the yeast,87%,followed by urine for 7.3%.In both of amphotericin B,making mould element sensitivity is the highest,about 100%,5-flucytosine sensitivity of 95% or more,to fluconazole and itraconazole sensitivity was the lowest.Conclusion:clinical samples in our sample really with white yeast bitter beads feed is given priority to,respiratory tract is yeast infection from the sample is the most common sites,most of the patients were low immunity to old people,the long-term use of antibiotics or immune inhibitors is infected fungal and drug reduced susceptibility to main reasons.