苦瓜基因组DNA提取方法的比较研究

Comparison of DNA Extraction Methods From Leaves of Momordica Charantia(Cucurbitaceae)

针对苦瓜富含多酚类化合物和多糖等物质的特点,以新鲜苦瓜叶片为材料,分别采用改良CTAB法、QIAGEN试剂盒、CTAB法提取基因组DNA,并对DNA进行纯度和浓度的检测,同时开展了SRAP验证.结果表明:使用改良CTAB法从苦瓜叶片中提取基因组DNA,其纯度高、质量好、相对分子量大,多酚类化合物和多糖等杂质去除比较完全.SRAP标记验证显示,条带清晰、多态性好.因此改良CTAB法适用于高质量提取苦瓜基因组DNA,并能满足SRAP等分子标记对DNA的要求.

Because of polysaccharides and polyphenolic and other interfering compounds in leaves of Momordica charan- tia （Cucurbitaceae）, the genomic DNA extraction of methods namly modified CTAB, Dneasy Kit and CTAB were com- pared. Then the quality and purified of extracted DNA was detected, and high quality DNA was measured by sequence- related amplified polymorphism （SRAP） markers. The results showed that modified CTAB moethod could be used to ex- tract DNA from leaves of M. charantia, with high qulity, high purify, high relative molecular mass and low interfering compounds. SPAP markers detection was also shown clear bands and good polymorphism. So, the modified CTAB meth- od was suitable for the extraction of genomic DNA from M. charantia, which was also available to further SRAP or other molecular markers study.