D型产气荚膜梭菌青海分离株ε毒素遗传变异分析

Genetic Variation of ε Toxin of Clostridium perfringens Type D Isolated from Qinghai

研究D型产气荚膜梭菌(Clostridium perfringens)青海分离株ε毒素基因的遗传变异特点,试验设计特异性引物扩增ε毒素基因,目的基因片段大小为888 bp。建立PCR反应体系并设置反应条件,扩增产物进行电泳检测、纯化、测定核苷酸序列,与参考序列进行同源比对分析。结果表明,目的基因扩增良好,分离菌株WC-epsilon-FL与菌株C60-3、NCTC 8346、Mukteshwar、AJ250956的核苷酸序列的同源性依次为99.9%、99.8%、98.9%、99.4%。

In order to research genetic variation of εtoxin gene of Clostridhtm pesfringens type D isolated from Qinghai, primers of which PCR product length was 888 bp was designed to amplify ε toxin gene. Amplification product was detected by electrophoresis, and purified and sequenced; then its homology with reference sequences was analyzed. The results indicated that toxin gene were amplified very well, nucleotide sequence homology rate was 99.9%,99.8% ,98.9% ,99.4% between Qinghai isolated strain and strain C60-3, strain NCTC 8346, strain Mukteshwar, strain AJ250956 ,respectively.