摘要
目的探讨杀伤细胞免疫球蛋白样受体(KIR)及其配体人类白细胞抗原(HLA)对肾移植患者术后发生急性排斥反应(AR)的作用。方法随机采集2009年3月-2012年3月实行肾移植的45对供受者,采用PCR-SSP方法进行HLA-Cw和KIR基因分型,然后依据受者移植后移植肾功能恢复状态分为AR组(16例)和非AR组(29例),分析供受者HLA-Cw和KIR基因型、受者KIR/供者HLA-Cw组合型与受者术后肾移植发生AR反应的相关性,籍此研究KIR及配体HLA-Cw对肾移植急性排斥反应的作用。结果在AR组中受者表达KIR2DL2/2DS2明显低于非AR组(P<0.05);非AR组中供受者KIR2DL2/HLA-Cw1、2DL3/HLA-Cw1和2DS2/HLA-Cw1组合型的匹配几率明显高于AR组(P<0.05)。结论特定的KIR基因(2DL2/2DS2)以及供受者KIR2DL2/HLA-Cw1、2DL3/HLA-Cw1和2DS2/HLA-Cw1组合型在异基因移植免疫应答中具有传导抑制信号的作用。
Objective To investigate the contribution of killer cell immunoglobulin - like receptors (KIR) and their human leukocyte antigen (HLA) class I ligands in the pathogenesis of acute rejection (AR) after kidney transplantation. Methods The donors and recipients of 45 kidney transplantations conducted in the Department of Organ Transplantation of Zhujiang Hospi- tal during March 2009 and March 2012 were enrolled in the study. According to the kidney function status of the recipients, the donor- recipient pairs were classfied into two groups, acute rejection group (AR, n= 16) and non- acute rejection group (n= 29). KIR and HLA- Cw genotyping were performed by polymerase chain reaction - sequence - specific priming (PCR - SSP) method. The possible associations of KIR genotypes and KIR/HLA combinations with acute rejection after kidney transplantation were explored. Results The expression rates of KIR2DL2/2DS2 in the AR group were significantly lower than those in the non- AR group (P 〈 0.05). The matching probabilities of KIR2DL2/HLA- Cwl, 2DL3/HLA - Cwl and 2DS2]HLA- Cwl combinations in the non - AR group were significantly higher than those in the AR group (P 〈 0.05). Conclusions Specific KIR genes (2DL2/2DS2) and KIR2DL2/HLA-Cwl, 2DL3/HLA-Cwl and 2DS2/HLA-Cwl combinations can conduct in- hibitory signals in the immune response of allogeneic transplantation.
出处
《实用预防医学》
CAS
2012年第8期1125-1127,共3页
Practical Preventive Medicine
基金
国家自然科学基金(81000312)
广东省自然科学基金(S2011010003948)
广东省医学科研基金(B2009151)