反相离子对高效液相色谱法测定盐酸林可霉素注射液中乙二胺四乙酸二钠的含量

Determination of Disodium Edetate in Lincomycin Hydrochloride Injection by Reversed-Phase Ion-Pair High Performance Liquid Chromatography

用反相离子对HPLC法测定盐酸林可霉素注射液中乙二胺四乙酸二钠的含量。Waters Symmetry C18柱,流动相:含12.5%甲醇和0.052%甲酸的水溶液(pH 3.5),检测波长为254nm,流速为1.0 mL·min-1,柱温为25℃。三批样品检测结果平均为0.001%。

This study is to establish a method for the determination of disodium edetate in lineomycin hydrochloride injection. The sample was separated on a Waters Symmetry Ct8 column, The mobile phase was an aqueous solution comprising 12.5% （v/v） methanol and 0.052% （v/v） formic acid （pH was adjusted to 3.5）. The detection was carried out at 254 nm and a flow rate of 1.0 mL·min^-1. The column temperature was 25℃. The average result was 0.001%.