HPLC法测定肠内营养乳剂中维生素C的含量

Determination of Vitamin C in Enteral Nutrition Emulsion by HPLC

建立肠内营养乳剂中维生素C的高效液相色谱测定法。用Merck LiChrospher100 NH2柱(250 mm×4.0 mm,5μm),0.02 mol.L-1磷酸二氢钾溶液-乙腈(27∶73)为流动相(pH为5.3),进行分离,检测波长为254 nm。该法线性范围为2.2868～7.5466μg.mL-1,精密度和平均回收率良好。

An HPLC method was established for the determination of vitamin C （VC） in enteral nutrition emulsion. The mobile phase consisted of 0.02 mol.L^-1 potassium dihydrogen phosphate-aeetonitrile （27：7.3, pH 5.3）. The determination was performed on a Merck LiChrospher 100 NH2 column （250 mm× 4.0 mm, 5μm）. The UV detection wavelength was set at 254 nm. The proposed method gained a linear range at 2.2868~7.5466 μg.mL^-1 （r=0.9999, n=5） with good accuracy and recovery.