摘要
目的:观察大蒜素对人肺腺癌细胞SLC-89增殖的影响。方法:不同浓度的大蒜素与SLC-89细胞共培养72 h,运用MTT法测定大蒜素的IC50。将IC50、IC50/2、IC50/4剂量的大蒜素与SLC-89细胞共培养72 h后,运用端粒重复序列扩增-酶联免疫吸附法分别测定细胞端粒酶活性,运用流式细胞技术测定细胞PCNA的表达率。加入等量生理盐水共培养作为对照组。结果:大蒜素能抑制SLC-89细胞的增殖,其IC50为79.8μg/mL,IC50和IC50/2剂量的大蒜素能显著抑制SLC-89细胞端粒酶的活性,IC50、IC50/2、IC50/4剂量的大蒜素都能显著抑制SLC-89细胞PCNA的表达。结论:大蒜素能抑制SLC-89细胞的增殖,其PCNA的表达比端粒酶活性更容易受到下调。
Objective: To investigate the effect of allicin on proliferation of human lung adenocarcinoma cell line (SLC-89). Methods: SLC-89 cells were cultured under different doses of allicin for 72 h, the 50 % inhibitory concentration (ICs0) was measured by MTY method. SLC-89 cells were then cultured with alfiein at dose IC50, IC50/2, IC2/50/4 for 72 h, respectively, telomerase activities of the treated cells were measured by Telomeric Repeat Amplification Protocol with ELISA (TRAP-ELISA), and expression of proliferating cell nuclear antigen (PCNA) of the treated cells were tested by flow eytometry. Results: Allicin inhibited proliferation of SLC-89 with IC50 of 79.8 μg/mL. A11icin at dose of IC50 and IC50/2 significantly inhibited telomerase activity of SLC-89 cells, and Alliein at dose of IC50 IC50/2 and IC50/4 significantly inhibited the expression of PCNA of SLC-89 cells. Conclusion: Allicin could inhibit proliferation of SLC-89 cells, compared to the telomerase activity, the expression of PCNA could be down-regulated more easily than by Allicin.
出处
《大理学院学报(综合版)》
CAS
2012年第6期32-34,共3页
Journal of Dali University
基金
云南省教育厅科研基金资助项目(2011Y440)