更年平调液对更年期大鼠心肌细胞保护及Bcl-2、Bax表达的影响

Effect of Gengnian Pingtiao liquid on expression of Bcl-2 and Bax in myocardial cell of rats with climacteric

[目的]探讨更年平调液对更年期大鼠心肌细胞保护作用的机制。[方法]选用鼠龄为11～15个月的自然更年期雌性SD大鼠,20只,随机分为2组,即更年平调液组(P组)、更年期对照组(M组)。另选鼠龄为4～6个月的SD大鼠,10只,为青年对照组(Y组)。药物干预第45天,采用免疫组织化学方法检测各组心肌组织中Bcl-2、Bax的表达,利用计算机图像分析技术测量各组心肌组织中Bcl-2、Bax的表达水平。[结果]更年平调液组Bcl-2、Bax的表达较更年期对照组差异有统计学意义(P<0.01),更年平调液组与青年对照组比较Bcl-2、Bax的表达差异无统计学意义(P>0.05)。[结论]更年平调液能抑制心肌细胞凋亡而对心肌细胞起着重要的保护作用。

[Objective] To explore of protective effects of Gengnian Pingtiao liquid on myocardial cell of rats with Climacteric. [Methods] The 20 SD rats of 11 to 15 months old with the natural climacteric were randomly＇divided into two groups： Gengnian Pingtiao liquid group （group P）, menopause control group （group M）. Another 10 SD rats, 4~6 month old were taken for young control group （group Y）. After experiment for 45 days, the average optical density （OD） and the rate of positive area of expression of Bcl-2 and Bax were analyzed by image analysis with HPIAS-2000 analysis system. [Results] The expression of Bcl-2 in group P was significantly higher than that in group M （P〈0.01）. No significant difference of expression of Bcl-2 was found between the group P and that in group Y （P〉0.05）. The expression of Bax in group P was significantly lower than that in group M （P〈0.01）. No significant difference was found in the expression of Bax between group P and that in group Y （P〉0.05）. [Conclusion] Gengnian Pingtiao liquid can inhibit the myocardial cell apoptosis and plays an important protective role for myocardial ceil.