结核杆菌L-型感染与肺癌组织类型的关系

Relationship between mycobacterium tuberculosis L-form infection and histological classification of lung carcinoma

目的调查肺癌组织中结核分枝杆菌L-型的感染状况,以分析结核分枝杆菌L-型感染相关肺癌的临床病理学特征。方法采用原位分子杂交技术检测结核分枝杆菌L-型MPB64基因片段的存在及定位。同时,用IK抗酸染色法检测标本中的结核分枝杆菌L-型。结果原位分子杂交检测石蜡包埋73例肺癌组织中MPB64基因片段在肺鳞癌、肺腺癌、细支气管肺泡癌、小细胞未分化癌中的阳性率分别为85.0%、73.5%、81.8%和87.5%,差异无统计学意义(P>0.05)。IK抗酸染色检测结核分枝杆菌L-型在肺鳞癌、肺腺癌、细支气管肺泡癌、小细胞未分化癌中的阳性率分别为70.0%、64.7%、63.6%和75.0%,差异无统计学意义(P>0.05)。结论结核分枝杆菌L-型感染在肺癌的形成过程中可能起到一定的作用,但是与癌组织的病理学类型无关。

Objective To survey the condition of mycobacterium tuberculosis L-form infection in lung carcinoma for analyzing the clinico-pathologic characteristics of mycobacterium tuberculosis L-form infection related-lung carcinoma. Meth- ods The formalin-fixed and paraffin-embedded tissue samples coming from surgically resected lung carcinomas of 73 cases were selected. The existing and location of MPB64 gene fragment of mycobacterium tuberculosis L-form in lung carcinoma tissue were detected by situ hybridization technique using MPB64 as probe, and meanwhile the mycobacterium tuberculosis L-form in it were detected by intensified kinyoun＇s （IK） anti-acid fast staining. Results The positive rates of MPB64 gene fragment by situ hybridization technique in squamous carcinoma, adenocarcinoma, bronchiole alveolus cancer and cellule no differentiation cancer were 85.0% ,73.5% ,81.8 % and 87.5% respectively, and they had no significant difference （P 〉 0.05 ）. The positive rates of myCobacterium tuberculosis L-form by IK anti-acid fast staining in squamous carcinoma, adeno- carcinoma, bronchiole alveolus cancer and cellule no differentiation cancer were 70.0%, 64.7%, 63.6% and 75.0% re- spectively, and they also had no significant difference （P 〉 0.05）. Conclusions Mycobacterium tuberculosis L-form infec- tion is likely to be involved in the occurrence or development of lung carcinoma, but it has no relation with histological classi- fication of lung carcinoma.