大连地区禽源大肠杆菌分离鉴定和耐药性调查

The Isolation,Verification and Drug-resistance Investigation of Avian E.coli in Dalian of Liaoning Province

本试验参照美国临床实验室标准化委员会(NCCLS)推荐的纸片扩散方法,用临床常用14种药物对109株禽源大肠杆菌,进行药敏试验和耐药数据分析。结果表明:临床分离109株大肠杆菌耐药性较大,多重耐药已经广泛存在。耐药比例较大药物:氟苯尼考耐药85株,78%;磷霉素钠71株,65%;恩诺沙星68株,62%。耐药菌株比例较小药物:头孢匹罗10株,9%、左旋氧氟沙星19株,17%、洛美沙星、头孢喹肟20株,18%,头孢曲松钠25株,23%,丁胺卡那霉素29株,27%。因此,目前,临床上长期、广泛、不按规定剂量导致耐药大肠杆菌菌株出现的概率较高。规范用药、轮换用药已成为兽医行业刻不容缓的问题。

Drug-resistance assays were undertaken by a disk diffusion method recommended by NCCLS, to analyze the resistance situations of 109 strains of isolated avian E. coli bacteria to 14 clinically popular drugs. The resulting data showed that the 109 strains could be categorized into two groups, depending on their anti-drug capacity. Of which, the high resistance group contained 85 strains （78%） resistant to florfenicol, 71 strains （65%） resistant to fosfomycin, and 68 strains （62%） resistant to enrofloxacin. In contrast, the low resistance group contained 10 strains （9%） resistant to cefpirome, 19 strains （17%） resistant to levofloxacin, and 20 strains （18%） resistant to lomefloxacin or cephalosporin quinolineoxime. Besides, there were also 25 strains （23%） and 29 strains （27%）, which showed low resistance to ceftriaxone sodium and amikacin, respectively. The results in total, suggested that the high rate emergence of drug-resistant bacteria strains was due to the fact that antibiotics are widely, long applied in the clinical therapies, sometimes even in a way of drug abuse. Thus, this called for a urgent need to regulate drug application in a rotation way and other proper ways in veterinary industry.