杜仲叶提取物抗氧化作用与总黄酮含量的相关性研究

Study on the Relativity of Antioxidant Activity and the Flavonoid Concentration of the Eucommia Leaf Extraction

采用二苯代苦味肼基自由基(DPPH.)和铁离子还原/抗氧化能力(FRAP)测定法,研究了杜仲叶提取物,芦丁的抗氧化活性,并同VC进行了比较。结果表明杜仲叶提取物具较强的抗氧化能力,抗氧化能力与总黄酮含量密切相关,在一定浓度范围内成线性关系,对DPPH.有较强的清除作用,半抑制量EC50为0.022mg/mL,各样品清除DPPH.能力为:VC>芦丁>杜仲叶提取液;在FRAP模型中,对铁离子的还原能力强度顺序为VC>杜仲叶提取物>芦丁;杜仲叶提取物抗氧化速度小于VC大于芦丁。

Comparing with VC,the antioxidant capacities of Eucommia leaf extraction and Rutin was analyzed by two methods,namely,1,1-diphenyl-2-picrylhydrazyl（DPPH·） radical scavenging and ferric reducing antioxidant power（FRAP） assay.The results showed that Eucommia leaf extraction had high antioxidant activity,and the antioxidant capacity of Eucommia leaf extraction was closely correlated with the concentration of flavonoids.The DPPH· free radical scavenging activity of Eucommia leaf extraction was lower than that of VC and Rutin,the EC50（50% the initial DPPH· concentration） of Eucommia leaf extraction was 0.022 mg/mL.But the ability to reduce ferric ions of Eucommia leaf extraction was lower than that of VC but higher than that of Rutin.The speed of antioxidant of Eucommia leaf extraction was higher than that of Rutin,and lower than that of VC.