溴酸钾-二甲酚橙催化褪色光度法测定虾仁中甲醛含量

Catalytic Spectrophotometric Determination of Formaldehyde in Shrimp Samples with Potassium Bromate-two Cresol Orange System

研究发现,在磷酸介质中,甲醛可以在一定温度下催化溴酸钾氧化二甲酚橙使其褪色,且褪色程度（ΔA）与甲醛的浓度在一定的范围内符合朗伯-比尔定律,据此建立了催化光度法测定甲醛含量的新方法。在最优的试验条件下,方法的线性范围为0.05~0.80μg/mL,检出限为0.013μg/mL,回收率在95%~110%之间。可用于虾仁中甲醛含量的测定,其效果令人满意。

A new catalytic spectrophotometric method was developed for the determination of formaldehyde based on its catalytic effect on the oxidation reactions of two cresol orange by potassium bromate in phosphoric acid medium.And the fading obeyed Lambert-Beer law with the concentration of formaldehyde in a certain range.Under the optimal condition,the linear range of the determination for formaldehyde was 0.05~0.80 μg/mL with a detection limit of 0.013 μg/mL.And the detection recovery was from 97.5%~105%.The method could be used to determine formaldehyde content in shrimp samples with satisfactory results.