上海地区汉族人高血压脑卒中与肾素系基因多态性的关系

Association between hypertensive cerebrovascular stroke and renin-angiotensin system gene polymorphism from Chinese cohort in Shanghai

目的 探讨血管紧张素原 (angiotensinogen,AGT)和血管紧张素转换酶 (angiotensinconverting enzyme,ACE)基因多态性与高血压性脑卒中的关系。方法 应用聚合酶链反应检测 2 5 7例单纯高血压患者 (essential hypertension,EH) ,和 2 18例高血压性脑卒中患者 (其中 131例出血性脑卒中 ,87例缺血性脑卒中 ) AGT基因 M2 35 T变异多态和 ACE基因插入 (I) /缺失 (D)多态。结果 缺血性脑卒中患者 ACE基因 DD基因型及 D等位基因频率明显高于出血性脑卒中和单纯性 EH(P<0 .0 5 ) ,基因型 DD/II的优势比分别为 3.2 5 (2 .2 0～ 4.79)及 2 .87(2 .0 3～ 4.0 6 ) ,等位基因 D/I的优势比分别是 1.83(1.38～2 .43)及 1.6 9(1.2 7～ 2 .2 4) ;而 AGT基因 Met2 35 Thr多态及联袂 DD+ TT/非 DD非 TT在各组之间差异均无显著性 (P>0 .0 5 )。结论 ACE基因 DD基因型和 D等位基因可能系高血压时缺血性脑卒中的危险因子。

Objective To evaluate the association between hypertensive cerebrovascular stroke and renin angiotensin system gene polymorphism from Chinese cohort. Methods The polymorphisms of angiotensinogen(AGT) and angiotensin converting enzyme(ACE) from 257 cases of simple essential hypertension(EH) and 218 cases of hypertensive stroke(131 hemorrhagic cases and 87 ischemic cases were detected by PCR RFLP. Results The frequencies of DD genotype and D allele of ACE gene in ischemic stroke were significantly higher than those in hemorrhagic stroke and EH, and the odds ratios(OR) of DD/II genotype were 3.25(2.20 4.79) and 2.87(2.03 4.06), while the OR of D/I allele were 1.83(1.38 2.43) and 1.69(1.27 2.24) respectively. Otherwise, there was no difference in frequency distribution of Met235Thr mutation polymorphism and DD+TT/non DD non TT combined among the three groups. Conclusion The I/D polymorphism of ACE gene may be one of the risk factors and susceptible genetic markers for ischemic stroke with essential hypertension in Chinese.