非小细胞肺癌中凋亡抑制基因Bcl-2的转录表达研究

Transcriptional expression of apoptosis suppression gene Bcl-2 in non-small cell lung carcinoma

目的 探讨 Bcl- 2基因过度转录表达在人非小细胞肺癌发生、发展中的作用。方法 采用Northern印迹杂交技术和非放射性地高辛标记检测系统 ,检测了 137份不同部位、不同性质的人肺组织中的 Bcl- 2基因的 m RNA表达。结果 肺组织中 Bcl- 2 m RNA的表达有从良性病变组织、远离癌灶的肺组织、癌旁组织到癌灶组织逐渐增高的趋势 ;其中 ,肺癌癌灶组织中 Bcl- 2 m RNA的表达较肺良性病变组织和远离癌灶的肺组织中的表达明显增强 (P<0 .0 1)。低分化肺癌组织中 Bcl- 2 m RNA表达强度明显高于中、高分化肺癌 (P<0 .0 5 ) ;癌转移淋巴结组织中 Bcl- 2 m RNA表达强度明显高于反应性增生淋巴结 (P<0 .0 5 ) ;鳞癌、腺癌、腺鳞癌组织中 Bcl- 2表达强度差异无显著性 (P>0 .0 5 )。结论 Bcl- 2基因的过度转录表达可能参与调节非小细胞肺癌的发生及转移过程。

Objective To investigate the role of Bcl 2 gene in oncogenesis and progression of non small cell lung carcinoma(NSCLC). Methods The mRNA expressions of Bcl 2 gene in a series of 137 pulmonary tissues collected at various sites and with different properties were studied with Northern hybridization and non radioactive digoxigenin labeling and detection system. Results According to the observations on benign lesions, non cancer tissues distant from tumour, para tumour tissues and cancer tissues, there was a tendency of mRNA expression increasing of Bcl 2 gene. Among them, Bcl 2 mRNA expression in lung cancer tissues was significantly increased, compared with that in benign lesions and tissues distant from tumour ( P <0.01). The Bcl 2 mRNA expression in lymph node with cancer metastasis was significantly higher than that in lymph node without cancer metastasis( P <0.05). The Bcl 2 mRNA expression was relative to cell differentiation of the cancer, but not to the histological classification.Conclusion Over expression of Bcl 2 gene might be involved in the oncogenesis and metastasis of NSCLC.