应用荧光原位杂交检测人喉癌中EGFR基因扩增

Detection of epidermal growth factor receptor gene amplification in human laryngeal carcinomas by means of fluorescence in situ hybridization

目的 检测人喉癌 Hep- 2细胞系和 5例喉癌组织中表皮生长因子受体 (epidermal growthfactor receptor,EGFR)基因扩增。方法 荧光原位杂交技术。结果 在 Hep- 2细胞系和 2例喉癌组织中期染色体和间期细胞核中 ,检测到明显的集中成簇和多个斑点分散排布的杂交信号 ,另 3例喉癌组织间期细胞核中杂交信号未见增强或数目增加。结论 以正常二倍体淋巴细胞染色体和间期细胞核为对照 ,在喉癌 Hep- 2细胞系和 2例喉癌组织中期染色体和间期细胞核中存在不同程度 EGFR基因扩增 ,扩增范围 2～8倍 ,另 3例喉癌组织细胞核中杂交信号强度和数目表明没有 EGFR基因扩增。证实荧光原位杂交方法可应用于定量检测细胞系和癌组织中的基因扩增 ,而且可检测单一细胞中的基因扩增。

Objective To detect epidermal growth factor receptor (EGFR) gene amplification in human laryngeal carcinoma Hep 2 cell line and laryngeal carcinoma tissues. Methods The technique of fluorescence in situ hybridization(FISH). Results The EGFR gene amplification of a laryngeal carcinoma Hep 2 cell line and 5 laryngeal carcinoma tissues were detected by FISH. In the metaphase chromosome and interphase nuclei of Hep 2 cell line and 2 laryngeal carcinoma tissues, distinct cluster and multiple dot signals were found. In the interphase nuclei of the other 3 laryngeal carcinoma tissues, no increase in the number or extent of the hybrid signals was found. Conclusion Compared with normal diploid cell, the EGFR gene amplification was observed at different levels ranging from 2 to 8 folds in metaphase chromosome and interphase nuclei of Hep 2 cell line and 2 laryngeal carcinoma tissues while no amplification was observed in the other 3 laryngeal carcinoma tissues. The results demonstrate that quantitative detection of amplified gene by FISH in the metaphase chromosome and interphase nuclei is useful for detection of laryngeal carcinoma tissues.