病证结合方证动物模型构建思路的探索

Construction of animal model of formula syndrome based on combination of disease and syndrome

目的:以生脉散为例,探讨病证结合方证动物模型构建中关键病理环节及造模方法的选择思路。方法:采用文献信息挖掘方法,利用频数分析、潜变量分析及信息挖掘方法。研究内容包括生脉散主治疾病谱、临床应用指征及与主治疾病相关的病理生理物质及过程。结果:生脉散主要用于冠心病、心力衰竭,慢性阻塞性肺病,休克、恶性肿瘤等疾病。其方证的一般特征包括乏力、口渴、纳差、神疲、消瘦等表现。生脉散主治疾病与内皮生长因子等生理病理物质及缺氧、肥胖、贫血等病理过程有关,其中缺氧符合该方证的一般特点。结论:纳差、神疲、消瘦等可以作为生脉散方证动物模型的目标症状表现,缺氧是该模型的关键病理环节,可以作为造模手段。

Objective：Taking the formula Shengmai Powder（SMP） for example,we discuss the methods of constructing animal model of formula syndrome based on combination of disease and syndrome.Methods：Information mining methods based on literature were adopted.We used frequency analysis,latent variable analysis,etc.to discover diseases spectrum,clinical indication and related pathophysiologic substances and processes of SMP.Results：The diseases in which SMP often used include coronary disease,heart failure,chronic obstructed pulmonary disease,shock,cancer,and so on.Weakness,thirst,inappetence,fatigued spirit and emaciation are the main clinical symptoms and signs of the formula syndrome,and SMP is closely related to many substances（vascular endothelial growth factor,for example） and processes（hypoxia,obesity and anemia,for example）.Conclusion：Weakness,fatigued spirit and emaciation can be the target symptoms and signs of animal model.Hypoxia is the key pathological taches and can be one of means of model construction.