辛伐他汀预处理对脓毒症大鼠凝血功能及肝脏C-反应蛋白的影响

Effects of simvastatin pretreatment on coagulation function and C - reactive protein of liver in septic rats

目的观察辛伐他汀预处理对脓毒症大鼠凝血功能及肝脏C-反应蛋白（CRP）的影响。方法采用盲肠结扎穿孔术（CLP）制备脓毒症大鼠模型。清洁级雌性Wistar大鼠80只被随机分为正常对照组（8只）、假手术组（6、24、48h各8只）、脓毒症模型组（6、24、48h各8只）和辛伐他汀预处理组（6、24、48h各8只），除正常对照组外，其余各组于CLP术前给予生理盐水或辛伐他汀20mg,／（kg·d）（溶于1mL蒸馏水），灌胃连续2周，各组分别于CLP术后6、24、48h后活杀动物8只，留取血液及肝组织标本。观察脓毒症大鼠-般表现并测定脓毒症大鼠白细胞计数（WBC）及血小板计数（PLT）、组织因子（TF）、纤溶酶原激活物抑制物-1（PAI-1）、肝脏CRP等指标。结果辛伐他汀预处理可以降低脓毒症大鼠TF、PAI-1水平，减少PLT消耗，同时降低肝脏组织CRP水平的表达。结论辛伐他汀预处理能够改善脓毒症大鼠的凝血功能；减轻肝脏炎症反应，保护肝脏功能。

Objective To observe the effects of simvastatin pretreatment on coagulation function and C - reactive protein of liver in septic rats. Methods In this experiment, we established the rat model of sepsis induced by cecal ligation and puncture. Eighty clean healthy female Wistar rats were chosen and divided into four groups at random： nomal group, sham group, sepsis model group and prevention group. Except normal group, saline or 20 mg/（ kg ·d） simvastatin（ 1 mL sterile water for injection） was injected to their stomaches for two weeks before CLP. Each group killed eight rats effectively at 6 h, 24 h, 48 h point and collected blood samples and liver tissue specimens. Monitor the general situation of rats in each group and examine white blood cell, platelet count, the changes of TF and PAl- 1 in serum and the level of CRP in liver tissue in each group. Results Pretreatment with simvastatin could inhibit the production of tissue factor and plasminogen activator inhibitor - 1 in the serum, decrease consumption of platelets. At the same time, it could descend the lever of C - reactive protein of liver. Conclusion Pretreatment with simvastatin could improve the coagulation function, alleviate the degree of liver inflammation and protect liver function.