睫状节神经营养因子在大鼠损伤坐骨神经中的表达变化

THE CHANGES ABOUT THE EXPRESSION OF CILIARY NEUROTROPHIC FACTOR IN INJURED SCIATIC NERVE OF RAT

目的 :探索坐骨神经损伤后睫状节神经营养因子 CNTF的表达变化。方法 :采用抗 CNTF抗体以Western Blot方法检测大鼠损伤两周坐骨神经远侧端和正常神经中 CNTF表达。结果 :正常神经组织中在 2 2 k D处有特异性 CNTF阳性反应条带 ,在损伤两周的坐骨神经远侧端中尚未出现 CNTF阳性反应条带。结论 :正常成年鼠坐骨神经中 CNTF表达处于一定的水平 ,神经损伤两周后的坐骨神经远侧端中 CNTF表达明显减少。

Objective: To probe CNTF changes in injuried sciatic nerve for 2wk.Methods: By anti-CNTF antibody, Western Blot was used to exam the different expression of CNTF in the distal stump of transected sciatic nerve for 2wk and normal sciatic nerve.Results: Special positive band was found around 22kD in normal nerve , no band in the distal stump of the injuried sciatic nerve. Conclusion:The expression of CNTF was in normal level in the normal sciatic nerve and decreased significantly in the distal stump of injuried nerve. [