免疫应答期间脑和淋巴器官内儿茶酚胺代谢的动态变化

METABOLIC ALTERATIONS OF CATECHOLAMINE IN THE BRAIN AND LYMPHOID ORGAN DURING IMMUNE RESPONSE

目的 :研究在免疫应答期间 ,脑和淋巴器官中儿茶酚胺代谢的动态变化 ,藉以了解免疫状态对中枢和外周儿茶酚胺能神经活动的影响。方法 :以绵羊红细胞作为抗原免疫 Sprague- Dawley大鼠 ,在免疫后的第 2、3、4、5、6、7d,应用高效液相色谱—电化学检测法测定大鼠下丘脑、海马、脑干和胸腺中去甲肾上腺素、肾上腺素、多巴胺和高香草酸的含量。结果 :1下丘脑去甲肾上腺素在免疫后第 4～ 7d都明显高于盐水对照组 ;海马内去甲肾上腺素只在免疫后第 4、5 d明显升高 ;胸腺中去甲肾上腺素在免疫后第 4、5 d明显降低。 2下丘脑肾上腺素在免疫后第 4～ 6 d显著增加 ;海马内肾上腺素在免疫后第 5、6 d明显增多 ;胸腺中肾上腺素明显增加发生在免疫后第 6、7d。 3下丘脑多巴胺在免疫后第 3、4d明显升高 ,而在第 5 d明显降低 ,高香草酸在免疫后第 2～ 5 d显著升高 ;海马内多巴胺在免疫后第 5～ 7d明显降低 ,而高香草酸在第 5～ 7d明显升高 ;胸腺中多巴胺只在免疫后第 6 d显著增加。4脑干中儿茶酚胺含量在免疫应答期间均无显著变化。结论 :免疫应答可能使下丘脑和海马中儿茶酚胺能神经的活动加强 ,对胸腺儿茶酚胺的影响可能依递质不同而作用不同 。

Objective: The metabolic alterations of catecholamine in the brain and lymphoid organ of rats during the immune response were investigated to know the effect of immune condition on the activity of catecholaminergic nerve in central and peripheral nervous system. Methods: Sprague-Dawley rats were immunized with sheep red blood cells (SRBC). During the days 2~7 after immunization, the content of noradrenaline (NA), adrenaline (A), dopamine (DA) and homovanilic acid (HVA) in the hypothalamus, hippocampus, brainstem and thymus of rats was determined by means of the high-performance liquid chromatography with electrochemical detection. Results: ①NA content in the hypothalamus and hippocampus significantly increased , the hypothalamic NA increase being during the days 4~7 and the hippocampal NA increase appearing on the days 4 and 5 after immunization. NA content in the thymus strikingly decreased on the days 4 and 5. ②A content in the hypothalamus, hippocampus and thymus markedly augmented. ③DA content in the hypothalamus significantly increased on the days 3 and 4, but dramatically decreased on the day 5 after immunization. HVA, DA metabolite, strikingly rose in the hypothalamus during the days 2~5. DA in the hippocampus markedly descended whereas HVA dramatically ascended during the days 5~7 after immunization. DA in the thymus markedly augmented only on the day 6. ④There were not significantly alterations of catecholamine content in the brainstem during the antibody response compared with the saline control. Conclusion: The immune response may enhance the activity of catecholaminergic nerve in the hypothalamus and hippocampus and may exert different impact on various catecholaminergic nerve in the thymus, but does not markedly affect the activity of catecholaminergic nerve in the brainstem. [