摘要
三角褐指藻(Phaeodactylum tricornutum)是开展微藻生物柴油研究的理想材料。克隆了内源fcp基因簇的多个调控序列(启动子、终止子),构建了包括fcpB启动子-bar基因-fcpA终止子、以及fcpA启动子-多克隆位点(MCS)-fcpA终止子两个表达盒的通用转化载体pfcpA-MCS/fcpB-Bar,其特征是以bar基因作为选择标记,MCS区方便插入一至多个目的基因。新载体可用于三角褐指藻的重组蛋白表达、或油脂代谢相关基因的功能验证和代谢调控研究。
Phaeodactylum tricornutum is an ideal material for biodiesel research. In this paper, several endogenous elements, i.e. promoters and terminators, from fcp (fucoxanthin, chlorophyll a/c-binding protein) gene clusters were cloned, and a transformation vector pfcpA-MCS/fcpB-bar including two expression cassettes which were fcpB promoter-bar gene-fcpA terminatiom and fcpA promoter-MCS (multiple cloning site)-fcpA terminatiom was constructed and characterized by using bar gene as selection marker. It carried a large MCS easy for insertion of one or more function genes. This vector will be used for lipid metabolic engineering for Phaeodactylum tricornutum.
出处
《生物学杂志》
CAS
CSCD
2012年第4期8-11,共4页
Journal of Biology
基金
973课题(2011CB200902)
863课题(2009AA10Z106)
山东省博士基金项目(2010BSB02009)
关键词
BAR基因
三角褐指藻
通用转化载体
bar gene
Phaeodactylum tricornutum
general transformation vector