内毒素血症大鼠肠道传输改变及一氧化氮作用

Reasearch of the Effect of Nitric Oxide on the Change of Intestinal Transit in Rats With Endofoxemia

目的 探讨内毒素血症状态下肠道传输改变及一氧化氮变化 ,为多器官功能衰竭的发生提供理论依据。方法 10 4只雄性Wistar大鼠 ,随机分为对照组和内毒素血症组 :肠道传输速度研究采用腹腔注射LPS 10mg/kg ;观察内毒素血症 3、6、12小时后大鼠血浆NO含量及空肠组织一氧化氮合酶 (NOS)活性 (包括iNOS,cNOS)变化 ,小肠铺片技术显示NOS阳性肌间神经丛 ,以及空肠免疫组化染色显示NOSⅡ阳性神经在肠道分布及内毒素血症后的变化。结果 内毒素血症后小肠传输速度加快 ,内毒素血症 3、6、12小时血浆NO、空肠NOS活性 (iNOS .cNOS)增强 ,并呈上升趋势 ,与对照组比较相差显著 (P <0 .0 1) ,肌间神经丛及空肠肠壁NOS阳性神经纤维及产物表达增多 ,与空肠组织含量相一致。结论 内毒素血症后肠道传输功能变化可能与NO有关。

Aim: To study the effect of nitric oxide (NO) on the change of intestinal transit during endotoxemia and to provide a basis for the mechanism of endotoxin translocation and multiple organ failure(MOF).Methods:The Wistar rats were divided into 2 groups: normal control and . endotoxemia group which were given LPS 10mg/kg intraperitonealy;Tissue levels of nitric oxide synthase(NOS) activity including constitude NOS(cNOS) and inducible NOS(iNOS) and serum levels of NO were measured at 3,6,12 hours postendotoxemia (to give LPS 10mg/kg intravenously).Histochemical and immohistochemical methods were used to evaluate the effect of endotoxemia on distribution of NOS immunoreactivity positive nerve fibre and product in jejuna.Results:Intestinal transit was rapid during endotoxemia;The tissue levels of NOS activity increased markdly on postendotoxemia. Increased NOS immunoreactive showed similar alteration with the tissue and serum levels of NO.Conclusion:The change of intesinal transit during endotoxemia possibly is asscoiated with NO.