雌激素改善大鼠记忆及促进海马BDNF蛋白表达上调

Estrogen to improve rat memory and hippocampal BDNF protein expression upregulation

目的探讨雌激素改善大鼠记忆与BDNF表达的关系。方法停止繁殖的15月龄雌性大鼠,体质量350～400g,随机分为治疗组和对照组,在颈部皮下分别注射相同剂量的苯甲酸雌二醇和生理盐水。采用Morris水迷宫检测大鼠记忆,大鼠海马BDNF、TrkB及mRNA表达采用RT-PCR检测,BDNF、TrkB及proBDNF蛋白表达采用Western-blot检测。结果治疗组大鼠潜伏期缩短,海马BDNF、TrkB、proBDNF表达上调(P<0.01)。结论雌激素改善大鼠记忆,可能与BDNF及相关蛋白表达上调有关。

Objective To explore the relation of estrogen on memory and expression of BDNF in hippocampus of postmenopaus al rats. Methods Rats retired from breeding for two reproductive cycles, 15-month-old, and 350-400 g weight, were divided into two groups by random assigned method. Before testing, estradiol benzoate and the same capacity of normal saline was injected subcuta- neouly on the cervical part of rats. memory of rats was detected by Morris water maze. RT-PCR and Western-blot wre respectively used to detect expression of BDNF mRNA, TrkB mRNA and protein of BDNF, TrkB and proBDNF hippocampus of postmenopausal rats. Results Compared with the control group, the rats of estrogen replacement therapy group showed a shorter escape latency （P〈0.05） Contrasted to the control group, expression of BDNF, TrkB and proBDNF protein in hippoeampus increased（P〈0.05）. Conclusion Memory is enhanced in the rats received estrogen replacement therapy group in postmenopausal rats,it maybe related with high expression of BDNF and relational protein.