UF-100尿沉渣分析仪显微镜复检规则的建立及评价

Establishment and evaluation the criteria of microscopic reexamination for UF-100

目的探讨UF-100尿沉渣计数与显微镜计数结果的符合情况及影响因素,制订UF-100尿沉渣分析的显微镜复检规则。方法采用双盲法,对507份新鲜尿样本进行UF-100尿沉渣分析和显微镜计数,制订出UF-100尿沉渣分析的显微镜复检规则,并统计复检率。结果显微镜评价真阳性率:红细胞为58%,白细胞为74.4%,上皮细胞为59.2%,管型为8.0%,酵母样细胞为84.6%。据统计结果,制订出本室UF-100尿沉渣分析的显微镜10条复检规则,应用该规则,统计2008、2009和2010年复检率分别为22.2%、27.8%和24.2%。结论 UF-100尿沉渣分析仪检测结果漏检率低,但也带来较高的假阳性,建立适合本实验室UF-100尿沉渣分析的显微镜复检规则是必要的。

Objective To institute the criteria of urine sediment microscopic reexamination, we investigated the coincidence rate and influencing factors between UF-100 and microscopic examination. Methods 507 freshly urine samples were analyzed by the routine microscopic examination with the methods of double-blind. Meanwhile,the criteria of microscopic reexamination of UF 100 were instituted. Results the real positive rate of microscopic evaluation：the positive coincidence rate of RBC, WBC, EC,Cast, yeast- like cells was 58 %, 74.4 %, 59.2 %, 8.0% and 84.6 %, respectively. Based on those statistics results, 10 criterion of microscopic re examination were developed in our clinical lab. The total reexamination rate in 2008,2009 and 2010 were 22.2 %, 27.8% and 24.2 respectively. Conclusion Since UF-100 could not accurately analyze urine sediment and cause the false positive results, UF-100 could be used as a rapid screening procedure for automated urinalysis. To increase the accuracy of UF 100,it is necessary for us to develop the criterion of urine sediment microscopic reexamination.