固相致敏红细胞吸附技术快速检测肝硬化患者血清中TIMP-1

SPASE for rapid detection of serum TIMP-1 in patients with liver cirrhosis

目的 建立一种改良固相致敏红细胞吸附技术(SPASE)用于快速检测肝硬化患者血清中 TIMP- 1.方法 用 TIMP- 1单克隆抗体包被微量血凝板 ,加热冲洗后加入待检血清标本 ,最后加入单克隆抗体致敏红细胞 .应用致敏红细胞作为指示系统 ,观察红细胞吸附状况来判定结果 .结果 SPASE法全过程仅需要 2 .5～ 3h,40 8例肝病患者血清检测 TIMP- 1结果为急性肝炎组检出阳性率 16 .4% ;慢性肝炎组阳性率为 33.3% ;肝硬化组阳性率为 73.6 % .结论 血清中 TIMP- 1的变化可作为肝纤维化较为有用的诊断指标 .SPASE法具有较高的敏感性、特异性和快速性 ,试剂用量少 ,且不需复杂的仪器设备 ,实验程序又简单 。

AIM To establish solid phase absorption to sensitized erythrocytes (SPASE) test for rapid detection of TIMP 1 in sera of patients with liver cirrhosis. METHODS Using antisensitized erythrocytes as indicator to replace enzyme or isotope, the result was judged by observing the agglutination of erythrocytes. A microtitre plate was coated with anti TIMP 1 McAb. After being heated and washed, the serum sample was added to the microtitre plate. The McAb sensitized erythrocytes were finally put into the microtitre plate. RESULTS The whole detection process by SPASE took only 2.5～3 h. In addition, it required only a small amount of reagent, with no special apparatus. Of 408 serum samples, 16.4% were higher in the group of acute hepatitis, 33.3% higher in the chronic hepatitis, and 73.6% higher in the liver cirrhosis group. CONCLUSION The serum TIMP 1 level is very useful in the assessment of liver fibrosis in chronic liver disease. SPASE is a very convenient and a specific technique for rapid detection of TIMP 1 from the serum, particularly for rural medical units.