乙型肝炎病毒DNA定量检测及意义

Quantitative detection of hepatitis B virus DNA and its significance

目的 检测血清中乙型肝炎病毒 DNA拷贝数 ,并了解 HBV感染的不同血清学指标组合相应的 HBV- DNA含量分布 ,以指导临床 .方法 采用 Ampli Sensor PCR定量方法 ,检测 2 0 8份不同临床类型血清标本的 HBV- DNA含量 ,再用EL ISA方法测定 HBV- M,统计不同免疫指标组合的 HBV-DNA平均含量 .结果 病毒量分为高、中、低三度 ,大于 10 7拷贝· m L- 1 为高滴度 ;10 7～ 10 5 拷贝· m L- 1 为中等滴度 ;10 5拷贝· m L- 1 以下为低滴度 .6 0例 HBs Ag(+) HBe Ag(+)HBc Ab(+)血清 ,HBV- DNA全部阳性 ,平均含量为 1.8× 10 8拷贝· m L- 1 ;48例 HBs Ag(+) HBe Ab(+) HBc Ab(+)血清 ,HBV- DNA平均含量为 6 .4× 10 6 拷贝· m L- 1 ;30例 HB-s Ag(+) HBc Ab(+)血清 ,HBV- DNA平均含量为 8.5× 10 5拷贝· m L- 1 ;13例 HBs Ab(+) HBe Ab (+) HBc Ab(+)血清 ,HBV- DNA平均含量为 2 .1× 10 5拷贝· m L- 1 .结论 定量 PCR可真实反应 HBV感染、复制及病程变化 。

AIM To detect the copy number of HBV-DNA in serum and to know the amount of HBV-DNA in different serological marker combinations. METHODS HBV-DNA was quantified by AmpliSensor PCR and HBV-M was detect-ed by ELISA in 208 sera with different clinical characteristics. The mean amount of HBV-DNA was counted in different serological marker combinations. RESULTS The quantity of HBV was divided into the high titer,the middle titer and the low titer. More than 10 7 copies·mL -1 were the high titer. The middle titer was between 10 7 copies·mL -1 and 10 5 copies·mL -1 . Less than 10 5 copies·mL -1 were the low titer. In 60 HBsAg(+) HBeAg(+) HBcAb(+) samples, HBV-DNA was positive, with 1.8×10 8 copies·mL -1 of HBV on average. In 48 HBsAg(+) HBeAb(+) HBcAb(+) samples, the average was 6.4×10 6 copies·mL -1 . In 30 HBsAg(+) HBcAb(+) samples, the average was 8.5×10 5 copies·mL -1 . In 13 HBsAb(+) HBeAb(+) HBcAb(+) samples, the amount was 2.1×10 5 copies·mL -1 . CONCLUSION Quantitative PCR can be used to monitor the true state of HBV infection, replication and the course of disease.