非小细胞肺癌中P-gp、p53、bcl-2蛋白表达及其相关性研究

Expression of MDR1 Gene Product P glycoprotein,p53 and bcl-2 Protein in Non small Cell Lung Cancer

目的 研究非小细胞肺癌 (NSCLC)中p5 3、bcl 2蛋白以及耐多药基因MDR 1产物P 糖蛋白 (P gp)的表达及相互关系 ,探讨它们在NSCLC发展、预后及耐药中的作用。方法 采用免疫组化S P法 ,对 60例NSCLC及其癌旁正常肺组织中 p5 3、bcl 2蛋白和P gp进行检测。 结果 p5 3、bcl 2蛋白及P gp在NSCLC中表达明显高于癌旁正常组织 (P <0 .0 1)。两者都与患者预后显著负相关 (P <0 .0 1) ,与组织分型无关 (P >0 .0 5 ) ,p5 3蛋白表达与分化程度负相关 (P <0 .0 5 )。三者之间 ,p5 3蛋白与bcl 2蛋白、p5 3蛋白与P gp无相关性 (P >0 .0 5 ) ,而P gp与bcl 2蛋白之间呈显著正相关。 结论 p5 3蛋白、bcl 2蛋白、P gp在NSCLC的发展过程中起了重要作用 ,可作为评价NSCLC患者预后的有效参数 ,P gp与bcl蛋白在NSCLC发展过程中有协调作用 ,可能存在一定的内在联系。

Objective To observe the expression of p53,bcl 2 protein and MDR1 P gp in non small cell lung cancer(NSCLC) and study their effects in NSCLC.Methods Sixty cases of NSCLC and tumor surrounding normal lung tissues were examined with immunohistochemistry technique.Results The positive rates of p53,bcl 2 protein and MDR1 P gp in NSCLC were 51.7%,61.7% and 63.3% respectively,which were higher than that in normal controls( P <0.01).The three kinds of protein expression were related with prognosis,and not related with histological subtype( P >0.05).The expression of p53 protein was related with differential degree of NSCLC.The expression of p53 protein was not related with bcl 2 and P gp,but the expression of P gp was related with bcl 2 in NSCLC.Conclusion The expression of p53,bcl 2 protein and P gp are correlated with the development of NSCLC,and have important value on predicting the prognosis of NSCLC.There may be some relationship between MDR1 and bcl 2 during the development of NSCLC.