摘要
目的 研究N - -甲基亚硝基脲 (MNU)诱发大白鼠膀胱肿瘤的作用。方法 以MNU为诱癌剂 ,进行大白鼠膀胱灌注 ,以光镜、扫描电镜、透射电镜等为观察手段 ,对其诱发的膀胱肿瘤进行观察。结果 每次灌注MNU2mg 只 ,每 2周 1次 ,共 4次 ,8周诱发膀胱肿瘤率 10 0 % ,其组织学形态及病理特征与人膀胱肿瘤十分相似。结论 MNU诱发的大白鼠膀胱肿瘤为一理想的动物模型。
Objectivc To study the experimental animal model of bladder tumors induced by MNU.Methods Bladder tumors were induced in female Wistar rats by intravesicular adminstration of the carcinogen,N-methyl -N-nitrosourea(MNU).The morphological alterations bladder epithelial cells were stuied by light micoscopy scanning and transmission electron microscopy.Results The administration way of a total dose of 8 mg on 4 fraction of 2 mg at 2- weekly intrvals produed a100%incidence of bladder toumor after 8 weeks.Histopathological characteristics of bladder tumor induced by MNU were basically similar to those of human bladder tumor.Conclusion Intravesicular administration of MNU in rats provided a useful model for experimental studies of bladder tumor.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2000年第4期241-243,共3页
Cancer Research on Prevention and Treatment
基金
山西省科学基金!资助项目 (9710 2 4)
关键词
化学致癌剂
MNU
甲基亚硝基脲
模型
膀胱肿瘤
Carcinogen N-mmethyl-noitrosourea
Experimental animal model
Bladder tumor
