系统性红斑狼疮BXSB小鼠骨髓B细胞系细胞的分化发育

Study on the differentiation and development of bone marrow B cell lineage cells from systemic lupus erythematosus BXSB mice

目的 :研究Yaa基因与骨髓B细胞系细胞的分化发育是否有相互关系。方法 :用Whitlock Witte法从骨髓细胞中培养纯化出B细胞系细胞 ,继而检测B细胞系细胞对脂多糖 (lipopolysaccharide,LPS)刺激后的表型、增殖反应和抗DNA抗体的产生以及对地塞米松诱导后的凋亡情况。结果 :用Whitlock Witte法成功地从骨髓细胞中培养分离出B细胞系细胞。经LPS刺激后 ,雌雄BXSB小鼠B细胞系细胞膜表面CD19抗原和膜表面Ig的分布相似 ,都出现较强的增殖反应和产生IgM类型的抗ssDNA和dsDNA抗体 ,但是对地塞米松诱导的凋亡作用不敏感。结论 :Yaa基因对BXSB小鼠骨髓中B细胞系细胞的分化发育无影响 ,提示Yaa基因仅在外周免疫器官的成熟B细胞中表达活性 ;并且BXSB小鼠骨髓中的自身反应性B细胞系细胞未被删除 ,可能是产生自身抗体的内在原因之一。

Objective: To obtain B cell lineage cells from the bone marrow of lupus BXSB mice and observe whether there is an interrelation between the Yaa gene and B cell lineage differentiation. Methods: B cell lineage cells were isolated with Whitlock Witte method, and their surface phenotype, proliferation, production of anti DNA antibody after response to lipopolysaccharide (LPS) and apoptosis status induced by dexamethasone were detected. Results:B cell lineage cells derived from male or female BXSB mice had identical distribution of CD19 and surface membrane Ig , proliferative response with similar intensity and produced approximate levels of IgM anti DNA antibodies after LPS stimulation. Besides , the B cell lineage cells were resistant to the Dex induced apoptosis. Conclusion: Yaa gene has no effects on the differentiation and development of the bone marrow B cell lineage cells in SLE BXSB mice, suggesting that its activity only appears in the peripheral mature B cells. The autoactive B cell lineage cells in the bone marrow of BXSB mice are not deleted, which maybe is one of the intrinsic reasons of producing autoantibodies.