鼻、鼻窦恶性肿瘤中EB病毒和人乳头状瘤病毒的检测

Detection of Epstein Barr virus and human papillomavirus in sinonasal malignant neoplasms

目的 :探讨 EB病毒和人乳头状瘤病毒 ( HPV)与鼻、鼻窦恶性肿瘤的关系。方法 :用聚合酶链反应( PCR)方法检测 3 2例鼻、鼻窦恶性肿瘤组织蜡块的 EB病毒和 HPV( HPV6、1 1、1 6、1 8、3 3 型 )基因 ,分析其与病理分型及 TNM分期的关系。结果 :3 2例中检出 EB病毒 12例 ( 3 7.5 % ) ,HPV2 1例 ( 65 .6% ) ,其中混合感染 6例 ,5例均未检出。与之对照的 10例鼻息肉中未检出 EBV和 HPV。TNM分期与 HPV和 EB病毒感染率无关 ,而 6例 EB病毒和 HPV混合感染均为 TNM分期 ～ 期 , ～ 期无一例混合感染。结论 :鼻、鼻窦恶性肿瘤不仅与 HPV感染有关 ,与 EB病毒感染也有一定关系。EBV和 HPV混合感染与鼻、鼻窦恶性肿瘤

Objective:To investigate the existance of Epstein-Barr virus (EBV) and papilloma virus(HPV) in sinonasal malignant neoplasms.Method:EBV and HPV (includingHPV 6,11,16,18,33 )gene were detected in paraffin embedded tissues with polymerase chain reaction(PCR) from 32 cases of sinonasal malignant neoplasms.Result:EBV was detected in 12( 37.5 %),HPV in 21( 65.6 %);The coinfection of EBV and HPV was found in 6 cases with stage Ⅲ~Ⅳ by TNM;10 cases of nasal polyps contained neither EBV nor HPV.Conclusion:Like HPV,there may be relationship between EBV and sinonasal malignant neoplasms.The coinfection of EBV and HPV is related to stage Ⅲ~Ⅳ by TNM of sinonasal malignant neoplasms.