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猪Ⅱ型圆环病毒江苏株全基因进化及ORF3蛋白的生物信息学分析 被引量：4

Bioinformatics Analysis of Whole Gene Evolution and ORF3 Coding Protein of Porcine Circovirus Type 2 Jiangsu Strain
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摘要 对NCBI已经登记的国内分离的PCV2江苏株(GQ358994.1)进行序列比对及进化分析,并对其ORF3编码蛋白的氨基酸序列进行生物信息学方法分析,对其组分、理化性质、跨膜结构域、二级结构、糖基化位点、磷酸化位点和B细胞抗原表位进行预测和推断。结果表明,PCV2江苏株与国内分离株序列同源性极高(>99%),ORF3蛋白无跨膜区域,二级结构中自由卷曲含量最高,为54.81%,不含有糖基化位点,但有8个磷酸化位点。综合分析得出,ORF3蛋白具有大量的抗原决定簇。通过对ORF3蛋白进行生物信息学方法分析,为PRRSV疫苗设计提供理论基础。 By bioinformatics methods and tools,the sequence of whole genes of PCV2 Jianssu strain（GQ358994.1）isolated in China registered by NCBI has been analyzed while the composition of amino acid sequences,molecular structure,physical and chemical characters,transmembrane helices,secondary structure,glycosylation site,phosphorylation site and antigen index of ORF3 coding protein were analyzed and predicted.The result shows that the sequences between Jiangsu strain and the other strains is fairly similar（〉99%）.ORF3 coding protein takes no transmembrane regions and the random coils covers 54.81%.It owns 7 phosphorylation sites while there are no glycosylation sites.The comprehensive analysis indicts the antigen index of ORF3 coding protein is fairly high.Theoretical foundation for the design of PCV2 vaccine could be provided by method of bioinformatics analysis.
作者 高歌 孙荡 茅翔
机构地区 南京农业大学动物医学院
出处 《山西农业科学》 2012年第8期881-885,共5页 Journal of Shanxi Agricultural Sciences
基金 南京农业大学大学生创新项目(SRT项目)(1004A04)
关键词 PCV2 进化分析 ORF3蛋白 生物信息学 PCV2； phylogenetic analysis； ORF3 coding protein； bioinformatics
分类号 S858.285.3 [农业科学—临床兽医学]
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参考文献17

  • 1夏应菊,訾占超,蔡林,韩雪,翟新验,田克恭,倪建强.猪细环病毒和猪圆环病毒2型混合感染状况的调查[J].畜牧兽医学报,2012,43(3):424-430. 被引量：12
  • 2Hamel A L,Lin L L,Nayar G P.Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs[J].J Virol,1998,72(6):5262-5267.
  • 3Sinha A,Lin K,Hemann M,et al.ORF1but not ORF2depen-dent differences are important for in vitro replication of PCV2in porcine alveolar macrophages singularly or coinfected with PRRSV[J].Vet Microbiol,2012,158(1/2):1-9.
  • 4Krakowka S,Allan G,Ellis J,et al.A nine-base nucleotide se quence in the porcine circovirus type2(PCV2)nucleocapsidgene determines viral replication and virulence[J].Virus Res,2012,164(1/2):90-99.
  • 5Chen H C,Chiou S T,Zheng J Y,et al.The nuclear localization signal sequence of porcine circovirus type2ORF2enhances in-tracellular delivery of plasmid DNA[J].Arch Virol,2011,156(5):803-815.
  • 6张秀珊,曹洪战,张丽青,刘涛,陈赛娟,孙继国.河北地区猪圆环病毒二型ORF2基因片段的克隆与原核表达[J].华北农学报,2008,23(4):51-54. 被引量：4
  • 7Karuppannan A K,Kwang J.ORF3of porcine circovirus2en-hances the in vitro and in vivo spread of the of the virus[J].Vi-rology,2011,410(1):248-256.
  • 8Li Wei,Kwang J,Jin Wang,et al.Porcine circovirus type2in-duces the activation of nuclear factor kappa B by IκBαdegradation[J].Virology,2008,378:177-184.
  • 9Chen X,Ren F,Hesketh J,et al.Reactive oxygen species regu-late the replication of porcine circovirus type2via NF-kappaB pathway[J].Virology,2012,426(1):66-72.
  • 10刘金鑫,孙孟娇,陈磊,王雪娇,银森森.猪圆环病毒研究进展[J].动物医学进展,2012,33(2):94-97. 被引量：16

二级参考文献50

  • 1胡慧,王亚宾,陈丽颖,张红英,杨高丰,崔保安.河南地区猪圆环病毒2型感染的血清流行病学调查[J].畜牧与兽医,2010,42(10):70-72. 被引量：16
  • 2洪健,周雪平.ICTV第八次报告的最新病毒分类系统[J].中国病毒学,2006,21(1):84-96. 被引量：87
  • 3杨增岐,杨泽晓,姜艳芬,祝卫国,王旭荣,史怀平.陕西省PCV2感染的血清学调查[J].西北农林科技大学学报（自然科学版）,2006,34(4):1-4. 被引量：11
  • 4陈溥言.兽医传染病学[M].5版.北京:中国农业出版社,2007:164-165.
  • 5Lefebvre D J, Costers S, et al. Antigenic differences among porcine circovirus type 2 strains, as demonstrated by the use of monoclonal antibodies[J]. J Gen Virol, 2008,89 : 177 - 187.
  • 6Mahe D,Blanchard P, et al. Differential recognition of ORF2 protein from type I and type 2 porcine circoviruses and identification of immunorelevant epitopes[J]. J Gen Virol, 2000,81:1815-1824.
  • 7Huang L P, Lu Y H, Wei Y W, et al. Identification of one critical amino acid that determines a eonformational neutralizing epitope in the capsid protein of porcine circovirus type 2 [J]. BMCMicrobiol, 2011, 11:1471-2180.
  • 8de Oloveria J X, Chitarra C S, de Paula D A J, et al. Occurrence of porcine circuvirus type-2 in slaughtered pigs in state of Mato Grosso [J]. Acta Sci Vet, 2009, 37(4): 363- 366.
  • 9Wang F, Guo X, Ge X, et al. Genetic variation analysis of Chinese strains of porcine circovirus type 2[J]. Virus Res, 2009, 145 (1): 151-156.
  • 10Ellis J A, Konoby C, West K H, et al. Lack of antibodies to porcine circovirus type-2 virus in bees and dairy catle and horses in western Canada [J]. Can Vet J, 2001, 42(6) : 461-464.

共引文献54

同被引文献27

  • 1杨晓农,郭万柱,徐志文,王新,殷华平,王小玉,龙虎.ORF3基因缺失对猪圆环病毒Ⅱ型感染复制能力的影响[J].中国兽医学报,2008,28(9):991-995. 被引量：9
  • 2Roghani M, Lassarre C, Zapf J, et al. Two insulin-like growth factor (IGF)-binding proteins are responsible for the selective affinity for IGF-Ⅱ of cerebrospinal fluid binding proteins [ J ]. J Clin Endocrinol Metab, 1991,73 : 658-666.
  • 3Ewton D Z,Florini J R. IGF binding proteins-4,-5 and -6 may play specialized roles during L6 myoblast prolifera- tion and differentiation [ J ]. Joural of Endocrinology, 1995,144 ( 3 ) :539-553.
  • 4Murphy L J. Insulin-like growth factor-binding proteins: Functional diversity or redundancy? [ J]. Journal of Mo- lecular Endocrinology, 1998,21 ( 2 ) :97-107.
  • 5Bach L A. IGFBP-6 five years on; not so ' forgotten ' ? [ J]. Growth Horm IGF Res,2005 ,15 :185-192.
  • 6Bach L A. Insulin-like growth factor binding protein 6: The " forgotten" binding protein [J]. Horn Metab Res, 1999,31 ( 2/3 ) :226-234.
  • 7Ficarro S B, McCleland M L, Stukenberg P T. Phospho- proteome analysis by mass spectrometry and its applica- tion to Saccharomyces cerevisiae[ J ]. Nature Biotechnolo- gy, 2002,20 ( 3 ) : 301-305.
  • 8Krupa A, Preethi G, Srinivasan N. Structural modes of stabilization of permissive phosphorylation sites in pro- tein kinases:Distinct strategies in Ser/Thr and Tyr kina- ses[ J]. J Mol Bio1,2004,339 : 1025-1039.
  • 9Neumann G, Bach L. The N-terminal disulfide linkages of human insulin-like growth factor-binding protein-6 (hlGFBP-6) and hIGFBP-1 are different as determined by mass spectrometry [ J]. J Biol Chem, 1999, 274: 14587-14594.
  • 10Hashimoto R, Fujiwara H, Fiigashihashi N, et al. Binding sites and binding properties of binary and ternary com- plexes of insulin-like growth factor-Ⅱ (IGF-Ⅱ ), IGF- binding protein-3 and acid-labile subunit [ J ]. J Bio/ Chem, 1997,272:27936-27942.

引证文献4

二级引证文献9

山西农业科学
2012年 第8期

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