广东省潮州市13750例妇女宫颈人乳头瘤病毒感染状况及基因型分析

Study on Human Papillomavirus Infection States and Genotypes of 13750Women in Chaozhou City , Guangdong Province

目的对广东省潮州市13750例妇女进行人乳头瘤病毒(HPV)筛查,探讨该地区宫颈HPV感染现状及基因亚型分布情况,为该地区预防HPV感染和防治宫颈癌提供科学依据。方法 2010年3月至7月在广东省潮州市采取整群抽样法,选择潮州市市区及其2个乡镇中符合纳入标准并完成研究的13750例受试者为研究对象。采用导流杂交基因芯片技术对HPV基因亚型进行分型检测(本研究遵循的程序符合本院人体试验委员会所制定的伦理学标准,得到该委员会批准,征得受试对象本人的知情同意,并与之签署临床研究知情同意书)。结果自13750例受试对象中,共计检出989例HPV呈阳性患者,感染率为7.2%。高危型HPV感染的前5位HPV基因亚型依次为HPV-52,-16,-58,-68及-33,感染率分别为27.50%,15.47%,10.82%,8.29%和6.77%。其中,单一、合并2种、3种、4种、5种及以上HPV亚型感染分别为752例(76.1%),156例(15.8%),53例(5.4%),18例(1.9%)及9例(0.9%)。低文化程度,低收入家庭、从事家务劳动及嗜烟、酒受试者的HPV感染率较高。结论 HPV基因亚型分布具有一定区域性,高危型HPV-52,-16和-58及合并1种HPV基因亚型感染在广东省潮州市常见。

Objective To provide the science foundation for preventing and treating uterine cervical cancer by investigating the status and genotypes of human papillomavirus（HPV） among 13 750 women in Chaozhou city,Guangdong Province. Methods From March to July 2010,a total of 13 750 married women were recruited from Chaozhou eity,Guangdong Province by the cluster sampling method. A total of 21 kinds of HPV genotypes infection were detected by flow through hybridization and gene chip technique among 13 750 women. This was a prospective observational epidemiology study with institutional ethics approval and written all participants consent. Results Among 13 750 women,989 cases were infected HPV, with the positive rate of 7.2%. The top five high-risk sub-types of HPV were HPV-52（27.50%） ,-16（15.47%） ,-58 （10.82%） ,-68（8.29%）and-33（6.77%）. There were 752 cases （76.1%） with single HPV type infection, 156 cases （15.8%） with double HPV sub-types infection, 53 cases （5. 4%） with triple HPV sub types infection, 18 cases （1. 9%） with fourfold HPV sub-types infection and 9 cases （0. 9%） with fivefold or much more HPV sub-types infection. There were some influence factors of HPV infection including low educational level, low-income families, home-workers and women who were addicted to alcohols or cigarettes. Conclusions High-risk, single genotype is the most common HPV infection types, and HPV- 52, 16 ,-58 are major subtypes with the regional distinction of HPV genotypes in Chaozhou city, Guangdong Province.