4种植物提取物对茶炭疽病菌的体外抑制作用

Inhibition of 4 plant extracts against Gloeosporium theae-sinesis in vitro

以丙酮作溶剂对芦柑皮、艾蒿、香樟叶和侧柏叶进行浸提;采用菌丝生长速率法测定各提取物对茶炭疽病菌菌丝的抑制作用,采用水琼脂培养法测定提取物对分生孢子萌发的抑制效果。结果表明:1 500μg/mL浓度下,各提取物对茶炭疽病菌菌丝均有抑制作用,其中芦柑皮96h的抑制率达到93.32%,艾蒿、香樟叶和侧柏叶96h的抑制率分别为61.10%、55.64%和48.20%,EC50分别为8.981、839.575、1 058.682μg/mL和1 429.094μg/mL;芦柑皮、艾蒿、香樟叶和侧柏叶提取物对分生孢子的抑制率分别为34.85%、85.54%、55.40%和59.68%,EC50分别为5 764.142、281.121、1 502.49μg/mL和969.369μg/mL。芦柑皮、艾蒿丙酮提取物具有开发植物源杀菌剂的潜力。

This study was to test the inhibitory effects of 4 plant extracts on Gloeosporium theae-sinesis Miyake in vitro. Citrus madurensis peels, Artemisia argyi plant, Cinnamomum camphora leaves and Platycladus orientalis leaves were extracted by acetone;Mycelium growth rate methods and water agar culture were applied to determine the inhibitory effects of these extracts against mycelium growth and spore germination of G. theae-sinesis, respec- tively. All extracts had effects on mycelium growth. After treatment for 96 h, the inhibition rates of C. maduren- sis peels, A. argyi plant, C. camphora leaves and P. orientalis leaves with dosage of 1 500 μg/mL were 93.32%, 61.10%, 55.64% and 48.20% , respectively, while their EC50 values were 8. 981 μg/mL, 839. 575 μg/mL, 1 058. 682 μg/mL and 1 429. 094 μg/mL, respectively; Their inhibition rates against spore germination were 34. 85%, 85.54%, 55.40% and 59.68%, respectively, and EC50 values were 5 764. 142, 281. 121, 1 502.49 μg/mL and 969. 369 μg/rnL, respectively. The acetone extracts of C. madurensis peels and A. argyi plant had great po- tential for the development of botanical fungicides.