光谱法研究三氯生与人类肿瘤相关DNA的相互作用

Study on the Interaction of Triclosan with Human Tumor Related DNA by Spectroscopic Methods

采用紫外光谱、荧光光谱和圆二色谱等多种光谱方法研究了三氯生与人类肿瘤相关DNA(抑癌基因p53 DNA和癌基因C-myc DNA)的相互作用,以期为从分子水平上评价三氯生与人类肿瘤发生的危害性提供科学依据。紫外减色效应、KI猝灭受到抑制及圆二色谱正负峰强度减弱等结果表明三氯生以弱嵌插模式与两种DNA发生作用,三氯生与DNA的结合位点数均小于1,以及DNA解链温度小幅升高等结果证实三氯生部分嵌插入DNA的沟区,影响了DNA的双螺旋结构。

Some investigations indicated that triclosan（TCS）has a genetic toxicity and would induce DNA damage.In this paper,a variety of spectroscopic methods,including UV spectrum,fluorescence spectrum and circular dichroism spectrum,were used to study the interaction of triclosan with human tumor related DNA（p53 DNA and C-myc DNA）,aiming at providing the scientific basis for triclosan＇s toxicity to human tumor at the molecular level.The UV experiments,KI quenching experiments and CD experiments suggested a weak intercalation between triclosan and DNA;Besides,melting experiments and fluorescence titration experiments also confirmed that triclosan interacts with DNA via partly interaction binding,thus affecting the double helix structure of DNA.