胶束毛细管电泳法同时测定消栓通络片中5种有效成分

Simultaneous Determination of Five Effective Components in Xiaoshuantongluo Tablet by Micellar Electrokinetic Capillary Chromatography

建立了胶束毛细管电泳法同时测定中药复方制剂消栓通络片中芦丁、丹参素、人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1含量的分析方法。研究了缓冲体系的浓度、添加剂种类、分离电压、进样时间对组分分离的影响,以60 mmol/L SDS-30 mmol/L Tris-10 mmol/L硼酸(含15%甲醇)作运行缓冲液,检测波长214 nm,5种组分在26 min内得到基线分离。芦丁、丹参素、人参皂苷Rb1、人参皂苷Rg1、三七皂苷R1的质量浓度分别在2.5～100、2.5～200、10～300、15～400、15～400 mg/L范围内与其峰面积呈良好的线性关系,检出限分别为0.3、0.9、3.0、5.0、6.0 mg/L。样品在低、中、高3个浓度下的加标回收率为93%～108%,相对标准偏差均不大于4.5%。该方法简便、快速,可用于实际样品检测。

A micellar electrokinetic capillary chromatographic method was established for the simultaneous determination of rutin,danshensu,ginsenoside Rb1,ginsenoside Rg1 and notoginsenoside R1 in Xiaoshuantongluo Tablet.The sample was extracted with methanol and separated on an uncoated fused silica capillary column（75 μm×60 cm,effective length 50 cm）,using 60 mmol/L SDS-30 mmol/L Tris-10 mmol/L H3BO3（containing 15% methanol） as running buffer,with applied voltage of 25 kV.The analytes were detected by UV detecter at 214 nm.Under the optimized conditions,a baseline separation of five components was achieved in 26 min.The calibration curves were linear in the ranges of 2.5-100 mg/L for rutin,2.5-200 mg/L for sodium danshensu,10-300 mg/L for ginsenoside Rb1,15-400 mg/L for ginsenoside Rg1 and notoginsenoside R1,with detection limits of 0.3,0.9,3.0,5.0,6.0 mg/L,respectively.The average recoveries of five components were between 93% and 108% with relative standard deviations not more than 4.5%.The method was simple and accurate,and was suitable for the routine analysis of five effective components in Xiaoshuantongluo Tablet.