期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

耐紫杉醇人肺腺癌A549细胞株中BRCA1基因启动子CpG岛甲基化的研究 被引量：4

The Study of CpG Island Methylation of BRCA1 Gene Promoter in a Taxol Induced Drug-resistant Human Lung Aadenocarcinoma Cell Line A549
下载PDF
导出
摘要 目的检测耐紫杉醇人肺腺癌A549细胞株(A549/Taxol)中BRCA1基因启动子CpG岛甲基化状态,探讨A549/Taxol细胞对紫杉醇的耐药机制。方法应用甲基化特异性聚合酶链反应(MSP)技术,检测耐紫杉醇人肺腺癌A549细胞株BRCA1基因启动子CpG岛甲基化状态。结果 A549/Taxol细胞存在BRCA1基因异常甲基化,呈部分甲基化。结论 A549/Taxol细胞存在BRCA1基因异常甲基化,可能是A549/Taxol细胞对紫杉醇耐药的机制之一。 Objective To detect the CpG island methylation status of BRCA1 gene promoter in the Taxol induced drug-resistant human lung adenocarcinoma cell line A549（A549/Taxol）,and to explore the resistance mechanisms of A549/Taxol.Methods A549/Taxol were examined CpG island methylation of BRCA1 gene promoter by methylation specific PCR（MSP）.Results IBRCA1 gene aberrant methylation of A549/Taxol cells is part of methylation.Conclusion BRCA1 gene aberrant methylation of A549/Taxol may be one of the resistance mechanisms of taxol in A549/Taxol.
作者 尹红英 王红兵
机构地区 徐州医学院 徐州医学院附属医院
出处 《实用癌症杂志》 2012年第4期337-338,共2页 The Practical Journal of Cancer
关键词 BRCA1基因 CPG岛 甲基化 甲基化特异性聚合酶链反应 BRCA1 gene CpG islands Methylation Methylation specific PCR
分类号 R734.2 [医药卫生—肿瘤]
  • 相关文献

参考文献7

  • 1Deaton AM, Bird A. CpG islands and the regulation of tran- scription[J]. Genes Dev ,2011,25 (10) : 1010.
  • 2Hong H, Yen HY, Brockmeyer A, et al. Changes in the mouse estrus cycle in response to BRCA1 inactivation suggest a po- tential link between risk factors for familial and s sporadic o- varian cancer[J]. Cancer Res ,2010,70 (1):221.
  • 3Morris JR, Boutell C, Keppler M, et al. The SUMO modifica- tion path-way is involved in the BRCA1 response to genotoxic stress[J]. Nature,2009,462 (7275): 886.
  • 4王红兵,王亚丽,刘德生,徐海洋,裴冬生,祖茂衡.尿多酸肽对人肺腺癌A549细胞的作用及人肺腺癌A549细胞株E-cadherin基因甲基化的研究[J].实用癌症杂志,2010,25(5):445-446. 被引量：9
  • 5Antill YC, Mitchell G, Johnson SA, et al. Gene methylation in breast ductalfluid from BRCA1 and BRCA2 mutation carriers[J]. Cancer Epidemiol Biomarkers Prey,2010,19 ( 1 ) :265.
  • 6Kleer CG. Carcinoma of the breast with medullary-like fea- tures: diagnostic challenges and relationship with BRCA1 and EZH2 functions[J]. Arch Pathol Lab Med, 2009,133 (11) :1822.
  • 7Cai FF, Kohler C, Zhang B, et al. Epigenetic therapy for breast cancer[J]. Int J M ol Sci, 2011,12 (7) :4465.

二级参考文献2

共引文献8

同被引文献26

  • 1宋传贵,胡震,袁文涛,狄根红,沈镇宙,黄薇,邵志敏.上海地区早发性乳腺癌患者BRCA1和BRCA2基因突变分析[J].中华医学杂志,2005,85(43):3030-3034. 被引量：26
  • 2付欣鸽,王振华,李锋,胡文浩,米开.热木,蒋金芳,李洪安.110例维吾尔族及汉族乳腺癌中BRCA1基因突变及P53蛋白表达的研究[J].中国组织化学与细胞化学杂志,2007,16(3):340-346. 被引量：6
  • 3Jemal A, Bray F, Center MM, et al. Global cancer statistics [J]. CA Cancer J Clin,2011,61 (2) :69 -90.
  • 4Siegel R, Naishadham D, Jemal A. Cancer statistics,2012 [J]. CA Cancer J Clin,2012,62(1) :10 -29.
  • 5Chen T. Overcoming drug resistance by regulating nuclear receptors [J]. Adv Drug Deliv Rev,2010,62(13) : 1257 - 1264.
  • 6Baguley BC. Multiple drug resistance mechanisms in cancer [ J ]. Mol biotechnol,2010,46 (3) :308 - 316.
  • 7Liu FS. Mechanisms of chemotherapeutic drug resistance in cancer therapy-a quick review [J]. Taiwan J Obstet Gyneco1,2009,48 ( 3 ) :239 - 244.
  • 8Brown T, Pilkington G, Bagust A, et al. Clinical effec- tiveness and cost-effectiveness of first-line chemotherapy for adult patients with locally advanced or metastatic non- small cell lung cancer: a systematic review and economic evaluation [ J]. Health Technol Assess,2013,17 (31) : 1 - 278.
  • 9Socinski MA, Morris DE, Masters CA, et al. Chemothera- peutic management of stage IV non-small cell lung cancer [ J ]. CHEST J,2003,123 ( 1 Suppl) : 226S - 243 S.
  • 10Melguizo C, Prados J, Luque R, et al. Modulation of MDR1 and MRP3 gene expression in lung cancer cells after paclitaxel and carboplatin exposure [ J ]. Int J Mol Sci,2012,13(12) :16624 - 16635.

引证文献4

二级引证文献11

实用癌症杂志
2012年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部