SOX7对胃癌MKN45细胞株体外迁移侵袭的影响

SOX7 inhibits migration and invasion of MKN45 cells in vitro

目的探讨转录调节因子SOX7对人胃癌MKN45细胞株体外迁移侵袭能力的影响。方法以人胃癌MKN45细胞株作为研究对象,用脂质体介导转染pIRES2-EGFP-SOX7重组质粒并建立稳定过表达SOX7的MKN45细胞株,用Real-timePCR和Western blot验证SOX7过表达的情况,并检测转染前后MKN45细胞增殖和侵袭能力的变化,并用Western blot检测Wnt/β-catenin信号通路下游关键因子MMP-9的表达情况。结果 Real-time PCR和Western blot证实转染后的MKN45细胞SOX7表达明显上调,转染组较对照组增殖和侵袭能力明显下降,且Wnt/β-catenin信号通路下游关键因子MMP-9表达明显下调。结论 SOX7在体外可以有效抑制人胃癌MKN45细胞株的增殖和侵袭,其机制可能是通过负性调控Wnt/β-catenin信号通路引起的。

Objective To investigate the effects of SOX7 on migration and invasion of MKN45 cells in vitro.Methods SOX7 overexpression in MKN45 cells by using pIRES2-EGFP-SOX7 plasmids.Real-time PCR and Western-blot were performed to detect the mRNA level and protein level of SOX7 in MKN45 with or without SOX7 overexpression.Proliferation and invasion assay were performed to examine the changes between MKN45 cells with SOX7 expression and control.Western-blot was performed to detect the change of MMP-9 expression between MKN45 cells with SOX7 expression and control.Results SOX7 was significantly upregulated in MKN45 after transfected.Proliferation and invasion were significantly decreased in MKN45 cells with SOX7 expression compared with control.The expression of MMP-9 was significantly decreased in MKN45 cells with SOX7 expression compared with control.Conclusion SOX7 could inhibit proliferation and invasion of MKN45 cells in vitro through blocking Wnt/β-catenin.